Preprint

PUBDB-2025-05594

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Intrinsic structure of lipoplexes embedded in polyelectrolyte multilayers

Krabbes, M.Extern* ; Kampik, V.Extern* ; Büttner, M. ; Kaysser, L. ; Schneck, E.Extern* ; Shen, C.Extern*DESY* ; Woelk, C. (Corresponding author)Extern*

2025

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Please use a persistent id in citations: doi:10.1101/2025.10.10.681465

Abstract: The functionalization of surfaces with therapeutically applicable nucleic acid carriers provides promising strategies in biomedical research to develop therapies which focus on local nucleic acid delivery. One such approach is the embedding of lipoplexes (LPXs) in polysaccharide-based polyelectrolyte multilayers (PEMs). PEMs based on hyaluronic acid and chitosan lead to efficient embedding of customized LPX connected with good biological activity. However, although quantitative evaluation demonstrates LPX embedding, information on the embedded LPXs has been missing. In this study we used synchrotron-based grazing-incidence small angle x-ray scattering to investigate the effects of the change in the chemical environment caused by the embedding into PEMs on the LPX’s internal mesoscopic structure. While the lamellar character of the LPXs was preserved, the repeat distance was affected by the embedding into polysaccharide-based coatings.

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Contributing Institute(s):

1. DOOR-User (DOOR ; HAS-User)
2. PETRA-D (FS-PETRA-D)

Research Program(s):

1. 633 - Life Sciences – Building Blocks of Life: Structure and Function (POF4-633) (POF4-633)
2. 6G3 - PETRA III (DESY) (POF4-6G3) (POF4-6G3)
3. DFG project G:(GEPRIS)396823779 - Entwicklung von Lipoplex-beladenen Modelloberflächen für die in situ Transfektion im Bereich der Knochenregeneration (396823779) (396823779)
4. FS-Proposal: R-20240669 (R-20240669) (R-20240669)

Experiment(s):

1. PETRA Beamline P08 (PETRA III)

Appears in the scientific report 2025

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