Journal Article

PUBDB-2019-00209

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Human Apolipoprotein A1 at Solid/Liquid and Liquid/Gas Interfaces

Dogan, S. (Corresponding author)Extern* ; Paulus, M. (Corresponding author)Extern* ; Forov, Y. ; Weis, C. ; Kampmann, M. ; Cewe, C. ; Kiesel, I. ; Degen, P. ; Salmen, P. ; Rehage, H. ; Tolan, M.Extern*

2018
ACS Washington, DC

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Please use a persistent id in citations: doi:10.1021/acs.jpcb.7b12481

Abstract: An X-ray reflectivity study on the adsorption behavior of human apolipoprotein A1 (apoA1) at hydrophilic and hydrophobic interfaces is presented. It is shown that the protein interacts via electrostatic and hydrophobic interactions with the interfaces, resulting in the absorption of the protein. pH dependent measurements at the solid/liquid interface between silicon dioxide and aqueous protein solution show that in a small pH range between pH 4 and 6, adsorption is increased due to electrostatic attraction. Here, the native shape of the protein seems to be conserved. In contrast, the adsorption at the liquid/gas interface is mainly driven by hydrophobic effects, presumably by extending the hydrophobic regions of the amphipathic helices, and results in a conformational change of the protein during adsorption. However, the addition of differently charged membrane-forming lipids at the liquid/gas interface illustrates the ability of apoA1 to include lipids, resulting in a depletion of the lipids from the interface.

Note: © American Chemical Society; Post referee fulltext in progress 2; Embargo 12 months from publication

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Contributing Institute(s):

1. DOOR-User (DOOR)

Research Program(s):

1. 899 - ohne Topic (POF3-899) (POF3-899)

Experiment(s):

1. DORIS Beamline BW1 (DORIS III)

Appears in the scientific report 2018

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Database coverage:
; Clarivate Analytics Master Journal List ; Current Contents - Physical, Chemical and Earth Sciences ; Ebsco Academic Search ; IF < 5 ; JCR ; NCBI Molecular Biology Database ; No Authors Fulltext ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Web of Science Core Collection

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