Journal Article

PUBDB-2014-03806

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Structural basis for organohalide respiration

Bommer, M.Extern* ; Kunze, C. ; Fesseler, J.Extern* ; Schubert, T. ; Diekert, G. ; Dobbek, H. (Corresponding Author)

2014
American Association for the Advancement of Science Washington, DC [u.a.]

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Please use a persistent id in citations: doi:10.1126/science.1258118

Abstract: Organohalide-respiring microorganisms can use a variety of persistent pollutants including trichloroethene (TCE) as terminal electron acceptors. The final two-electron transfer step in organohalide respiration is catalyzed by reductive dehalogenases. Here we report the x-ray crystal structure of PceA, an archetypal dehalogenase from Sulfurospirillum multivorans, as well as structures of PceA in complex with TCE and product analogs. The active site harbors a deeply buried norpseudo-B12 cofactor within a nitroreductase fold, also found in a mammalian B12 chaperone. The structures of PceA reveal how a cobalamin supports a reductive haloelimination exploiting a conserved B12-binding scaffold capped by a highly variable substrate-capturing region.

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Contributing Institute(s):

1. DOOR-User (DOOR)
2. Experimentebetreuung PETRA III (FS-PE)

Research Program(s):

1. PETRA Beamline P11 (POF2-54G14) (POF2-54G14)

Experiment(s):

1. PETRA Beamline P11 (PETRA III)

Appears in the scientific report 2014

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Database coverage:
; BIOSIS Previews ; Current Contents - Agriculture, Biology and Environmental Sciences ; Current Contents - Life Sciences ; Current Contents - Physical, Chemical and Earth Sciences ; JCR ; NCBI Molecular Biology Database ; National-Konsortium ; Nationallizenz ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Thomson Reuters Master Journal List ; Web of Science Core Collection ; Zoological Record

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