TY  - JOUR
AU  - Banaszak, K.
AU  - Mechin, I.
AU  - Obmolova, G.
AU  - Oldham, M.
AU  - Chang, S. H.
AU  - Ruiz, T.
AU  - Radermacher, M.
AU  - Kopperschlaeger, G.
AU  - Rypniewski, W.
AU  - DESY
TI  - The crystal structures of eukaryotic phosphofructokinases from baker's yeast and rabbit skeletal muscle.
JO  - Journal of molecular biology
VL  - 407
SN  - 0022-2836
CY  - Amsterdam [u.a.]
PB  - Elsevier
M1  - PHPPUBDB-19271
SP  - 284-297
PY  - 2011
AB  - Phosphofructokinase 1 (PFK) is a multisubunit allosteric enzyme that catalyzes the principal regulatory step in glycolysis-the phosphorylation of fructose 6-phosphate to fructose 1,6-bisphosphate by ATP. The activity of eukaryotic PFK is modulated by a number of effectors in response to the cell's needs for energy and building blocks for biosynthesis. The crystal structures of eukaryotic PFKs-from Saccharomyces cerevisiae and rabbit skeletal muscle-demonstrate how successive gene duplications and fusion are reflected in the protein structure and how they allowed the evolution of new functionalities. The basic framework inherited from prokaryotes is conserved, and additional levels of structural and functional complexity have evolved around it. Analysis of protein-ligand complexes has shown how PFK is activated by fructose 2,6-bisphosphate (a powerful PFK effector found only in eukaryotes) and reveals a novel nucleotide binding site. Crystallographic results have been used as the basis for structure-based effector design.
KW  - Animals
KW  - Binding Sites: genetics
KW  - Crystallography
KW  - Eukaryota: enzymology
KW  - Fructosephosphates: metabolism
KW  - Glycolysis: genetics
KW  - Models, Molecular
KW  - Muscle, Skeletal: enzymology
KW  - Phosphofructokinase-1: chemistry
KW  - Phosphofructokinase-1: genetics
KW  - Phosphofructokinase-1: metabolism
KW  - Phosphofructokinases: chemistry
KW  - Phosphofructokinases: genetics
KW  - Phosphofructokinases: metabolism
KW  - Rabbits
KW  - Saccharomyces cerevisiae: enzymology
KW  - Saccharomyces cerevisiae: genetics
KW  - Fructosephosphates (NLM Chemicals)
KW  - fructose-6-phosphate (NLM Chemicals)
KW  - Phosphofructokinases (NLM Chemicals)
KW  - Phosphofructokinase-1 (NLM Chemicals)
LB  - PUB:(DE-HGF)16
C6  - pmid:21241708
UR  - <Go to ISI:>//WOS:000288925100007
DO  - DOI:10.1016/j.jmb.2011.01.019
UR  - https://bib-pubdb1.desy.de/record/95857
ER  -