TY  - JOUR
AU  - Laib, S.
AU  - Fellah, B. H.
AU  - Fatimi, A.
AU  - Quillard, S.
AU  - Vinatier, C.
AU  - Gauthier, O.
AU  - Janvier, P.
AU  - Petit, M.
AU  - Bujoli, B.
AU  - Bohic, S.
AU  - Weiss, P.
AU  - DESY
TI  - The in vivo degradation of a ruthenium labelled polysaccharide-based hydrogel for bone tissue engineering
JO  - Biomaterials
VL  - 30
SN  - 0142-9612
CY  - Amsterdam [u.a.]
PB  - Elsevier Science
M1  - PHPPUBDB-8264
SP  - 1568-1577
PY  - 2009
AB  - In this paper we report a new method that permitted for the first time to selectively track a polysaccharide-based hydrogel on bone tissue explants, several weeks after its implantation. The hydrogel, which was developed for bone healing and tissue engineering, was labelled with a ruthenium complex and implanted into rabbit bone defects in order to investigate its in vivo degradation. 1, 2, 3 and 8 weeks after surgery, the bone explants were analyzed by synchrotron X-ray microfluorescence, infrared mapping spectroscopy, scanning electron microscopy, and optical microscopy after histological coloration. The results showed that the labelled polysaccharide-based hydrogel was likely to undergo phagocytosis that seemed to occur from the edge to the center of the implantation site up to at least the 8th week.
KW  - Absorbable Implants
KW  - Animals
KW  - Biocompatible Materials: metabolism
KW  - Bone and Bones: drug effects
KW  - Bone and Bones: metabolism
KW  - Calcium Phosphates: metabolism
KW  - Cell Line
KW  - Cell Survival: drug effects
KW  - Cells, Cultured
KW  - Ceramics: metabolism
KW  - Chondrocytes: cytology
KW  - Chondrocytes: drug effects
KW  - Cross-Linking Reagents: pharmacology
KW  - Femur: pathology
KW  - Femur: ultrastructure
KW  - Humans
KW  - Hydrogel: metabolism
KW  - Methylcellulose: analogs & derivatives
KW  - Methylcellulose: chemistry
KW  - Methylcellulose: metabolism
KW  - N-Acetylneuraminic Acid: chemistry
KW  - N-Acetylneuraminic Acid: metabolism
KW  - Osteogenesis: drug effects
KW  - Prosthesis Implantation
KW  - Rabbits
KW  - Ruthenium: metabolism
KW  - Time Factors
KW  - Tissue Engineering
KW  - Biocompatible Materials (NLM Chemicals)
KW  - Calcium Phosphates (NLM Chemicals)
KW  - Ceramics (NLM Chemicals)
KW  - Cross-Linking Reagents (NLM Chemicals)
KW  - N-Acetylneuraminic Acid (NLM Chemicals)
KW  - Hydrogel (NLM Chemicals)
KW  - Ruthenium (NLM Chemicals)
KW  - hypromellose (NLM Chemicals)
KW  - Methylcellulose (NLM Chemicals)
KW  - calcium phosphate (NLM Chemicals)
LB  - PUB:(DE-HGF)16
C6  - pmid:19101030
UR  - <Go to ISI:>//WOS:000263402500013
DO  - DOI:10.1016/j.biomaterials.2008.11.031
UR  - https://bib-pubdb1.desy.de/record/87279
ER  -