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000081236 1001_ $$aMokranjac, Dejana$$b0
000081236 245__ $$aStructure and function of Tim14 and Tim16, the J and J-like components of the mitochondrial protein import motor
000081236 260__ $$aHoboken, NJ [u.a.]$$bWiley$$c2006
000081236 300__ $$a4675-4685
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000081236 440_0 $$aEMBO J.$$v25$$x0261 - 4189
000081236 500__ $$3Converted on 2013-05-30 11:20
000081236 500__ $$3Converted on 2013-06-21 19:20
000081236 520__ $$aThe import motor of the mitochondrial translocase of the inner membrane (TIM23) mediates the ATP-dependent translocation of preproteins into the mitochondrial matrix by cycles of binding to and release from mtHsp70. An essential step of this process is the stimulation of the ATPase activity of mtHsp70 performed by the J cochaperone Tim14. Tim14 forms a complex with the J-like protein Tim16. The crystal structure of this complex shows that the conserved domains of the two proteins have virtually identical folds but completely different surfaces enabling them to perform different functions. The Tim14-Tim16 dimer reveals a previously undescribed arrangement of J and J-like domains. Mutations that destroy the complex between Tim14 and Tim16 are lethal demonstrating that complex formation is an essential requirement for the viability of cells. We further demonstrate tight regulation of the cochaperone activity of Tim14 by Tim16. The first crystal structure of a J domain in complex with a regulatory protein provides new insights into the function of the mitochondrial TIM23 translocase and the Hsp70 chaperone system in general.
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000081236 650_7 $$00$$2NLM Chemicals$$aMembrane Transport Proteins
000081236 650_7 $$00$$2NLM Chemicals$$aMitochondrial Membrane Transport Proteins
000081236 650_7 $$00$$2NLM Chemicals$$aMitochondrial Proteins
000081236 650_7 $$00$$2NLM Chemicals$$aMolecular Motor Proteins
000081236 650_7 $$00$$2NLM Chemicals$$aPam16 protein, S cerevisiae
000081236 650_7 $$00$$2NLM Chemicals$$aProtein Subunits
000081236 650_7 $$00$$2NLM Chemicals$$aSaccharomyces cerevisiae Proteins
000081236 650_7 $$00$$2NLM Chemicals$$aTim14 protein, S cerevisiae
000081236 650_2 $$2MeSH$$aAmino Acid Sequence
000081236 650_2 $$2MeSH$$aCrystallography, X-Ray
000081236 650_2 $$2MeSH$$aDimerization
000081236 650_2 $$2MeSH$$aEvolution, Molecular
000081236 650_2 $$2MeSH$$aMembrane Transport Proteins: chemistry
000081236 650_2 $$2MeSH$$aMembrane Transport Proteins: metabolism
000081236 650_2 $$2MeSH$$aMitochondrial Membrane Transport Proteins
000081236 650_2 $$2MeSH$$aMitochondrial Proteins: chemistry
000081236 650_2 $$2MeSH$$aMitochondrial Proteins: metabolism
000081236 650_2 $$2MeSH$$aModels, Molecular
000081236 650_2 $$2MeSH$$aMolecular Motor Proteins: chemistry
000081236 650_2 $$2MeSH$$aMolecular Motor Proteins: metabolism
000081236 650_2 $$2MeSH$$aMolecular Sequence Data
000081236 650_2 $$2MeSH$$aProtein Structure, Secondary
000081236 650_2 $$2MeSH$$aProtein Structure, Tertiary
000081236 650_2 $$2MeSH$$aProtein Subunits: chemistry
000081236 650_2 $$2MeSH$$aProtein Subunits: metabolism
000081236 650_2 $$2MeSH$$aProtein Transport
000081236 650_2 $$2MeSH$$aSaccharomyces cerevisiae: chemistry
000081236 650_2 $$2MeSH$$aSaccharomyces cerevisiae: metabolism
000081236 650_2 $$2MeSH$$aSaccharomyces cerevisiae Proteins: chemistry
000081236 650_2 $$2MeSH$$aSaccharomyces cerevisiae Proteins: metabolism
000081236 650_2 $$2MeSH$$aSequence Alignment
000081236 650_2 $$2MeSH$$aStructure-Activity Relationship
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000081236 7001_ $$0P:(DE-H253)PIP1007425$$aBourenkov, Gleb$$b1
000081236 7001_ $$aHell, Kai$$b2
000081236 7001_ $$aNeupert, Walter$$b3
000081236 7001_ $$0P:(DE-HGF)0$$aGroll, Michael$$b4$$eCorresponding author
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