TY  - EJOUR
AU  - Amin, Muhamed
AU  - Hartmann, Jean-Michel
AU  - Samanta, Amit K.
AU  - Küpper, Jochen
TI  - Laser-induced alignment of nanoparticles and macromolecules for single-particle-imaging applications
IS  - arXiv:2306.05870
M1  - PUBDB-2024-01943
M1  - arXiv:2306.05870
PY  - 2023
N1  - Please link this preprint with journal entry https://bib-pubdb1.desy.de/record/585553, when published
AB  - Laser-induced alignment of particles and molecules was long envisioned to support three-dimensional structure determination using single-particle imaging with x-ray free-electron lasers [PRL 92, 198102 (2004)]. However, geometric alignment of isolated macromolecules has not yet been demonstrated. Using molecular modeling, we analyzed and demonstrated how the alignment of large nanorods and proteins is possible with standard laser technology, and performed a comprehensive analysis on the dependence of the degree of alignment on molecular properties and experimental details. Calculations of the polarizability anisotropy of about 150,000 proteins yielded a skew-normal distribution with a location of 1.2, which reveals that most of these proteins can be aligned using appropriate, realistic experimental parameters. Moreover, we explored the dependence of the degree of alignment on experimental parameters such as particle temperature and laser-pulse energy.
LB  - PUB:(DE-HGF)25
DO  - DOI:10.3204/PUBDB-2024-01943
UR  - https://bib-pubdb1.desy.de/record/607607
ER  -