000578866 001__ 578866
000578866 005__ 20250715173014.0
000578866 0247_ $$2doi$$a10.1038/s41589-022-01226-w
000578866 0247_ $$2ISSN$$a1552-4450
000578866 0247_ $$2ISSN$$a1552-4469
000578866 0247_ $$2datacite_doi$$a10.3204/PUBDB-2023-00996
000578866 0247_ $$2altmetric$$aaltmetric:141818274
000578866 0247_ $$2pmid$$apmid:36702959
000578866 0247_ $$2WOS$$aWOS:000922974200003
000578866 0247_ $$2openalex$$aopenalex:W4318071640
000578866 037__ $$aPUBDB-2023-00996
000578866 041__ $$aEnglish
000578866 082__ $$a570
000578866 1001_ $$0P:(DE-H253)PIP1092788$$aCaserta, Giorgio$$b0$$eCorresponding author
000578866 245__ $$aStepwise assembly of the active site of [NiFe]-hydrogenase
000578866 260__ $$aBasingstoke$$bNature Publishing Group$$c2023
000578866 3367_ $$2DRIVER$$aarticle
000578866 3367_ $$2DataCite$$aOutput Types/Journal article
000578866 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article$$bjournal$$mjournal$$s1727083146_1407254
000578866 3367_ $$2BibTeX$$aARTICLE
000578866 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000578866 3367_ $$00$$2EndNote$$aJournal Article
000578866 500__ $$aFunding:Einstein Foundation Berlin: grant number EVF-2016-277;SPP 1927 “Iron Sulfur for Life”: grants: DE1877/1-1 and 311062227;Use of the Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, was supported by the U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences under Contract No. DE-AC02-76SF00515
000578866 520__ $$a[NiFe]-hydrogenases are biotechnologically relevant enzymes catalyzing the reversible splitting of H$_2$ into 2e$^−$ and 2H$^+$ under ambient conditions. Catalysis takes place at the heterobimetallic NiFe(CN)$_2$(CO) center, whose multistep biosynthesis involves careful handling of two transition metals as well as potentially harmful CO and CN$^−$ molecules. Here, we investigated the sequential assembly of the [NiFe] cofactor, previously based on primarily indirect evidence, using four different purified maturation intermediates of the catalytic subunit, HoxG, of the O$_2$-tolerant membrane-bound hydrogenase from Cupriavidus necator. These included the cofactor-free apo-HoxG, a nickel-free version carrying only the Fe(CN)$_2$(CO) fragment, a precursor that contained all cofactor components but remained redox inactive and the fully mature HoxG. Through biochemical analyses combined with comprehensive spectroscopic investigation using infrared, electronic paramagnetic resonance, Mössbauer, X-ray absorption and nuclear resonance vibrational spectroscopies, we obtained detailed insight into the sophisticated maturation process of [NiFe]-hydrogenase.
000578866 536__ $$0G:(DE-HGF)POF4-632$$a632 - Materials – Quantum, Complex and Functional Materials (POF4-632)$$cPOF4-632$$fPOF IV$$x0
000578866 536__ $$0G:(DE-HGF)POF4-6G3$$a6G3 - PETRA III (DESY) (POF4-6G3)$$cPOF4-6G3$$fPOF IV$$x1
000578866 536__ $$0G:(DE-H253)I-20200452$$aFS-Proposal: I-20200452 (I-20200452)$$cI-20200452$$x2
000578866 536__ $$0G:(GEPRIS)390540038$$aDFG project 390540038 - EXC 2008: Unifying Systems in Catalysis "UniSysCat" (390540038)$$c390540038$$x3
000578866 588__ $$aDataset connected to CrossRef, Journals: bib-pubdb1.desy.de
000578866 693__ $$0EXP:(DE-H253)P-P01-20150101$$1EXP:(DE-H253)PETRAIII-20150101$$6EXP:(DE-H253)P-P01-20150101$$aPETRA III$$fPETRA Beamline P01$$x0
000578866 7001_ $$0P:(DE-HGF)0$$aHartmann, Sven$$b1
000578866 7001_ $$0P:(DE-H253)PIP1085616$$aVan Stappen, Casey$$b2
000578866 7001_ $$0P:(DE-HGF)0$$aKarafoulidi-Retsou, Chara$$b3
000578866 7001_ $$0P:(DE-H253)PIP1100547$$aLorent, Christian$$b4
000578866 7001_ $$0P:(DE-HGF)0$$aYelin, Stefan$$b5
000578866 7001_ $$0P:(DE-HGF)0$$aKeck, Matthias$$b6
000578866 7001_ $$0P:(DE-HGF)0$$aSchoknecht, Janna$$b7
000578866 7001_ $$0P:(DE-H253)PIP1016663$$aSergueev, Ilya$$b8$$udesy
000578866 7001_ $$0P:(DE-HGF)0$$aYoda, Yoshitaka$$b9
000578866 7001_ $$0P:(DE-HGF)0$$aHildebrandt, Peter$$b10
000578866 7001_ $$0P:(DE-HGF)0$$aLimberg, Christian$$b11
000578866 7001_ $$0P:(DE-H253)PIP1015325$$aDeBeer, Serena$$b12
000578866 7001_ $$0P:(DE-H253)PIP1100480$$aZebger, Ingo$$b13
000578866 7001_ $$00000-0002-4141-7836$$aFrielingsdorf, Stefan$$b14$$eCorresponding author
000578866 7001_ $$00000-0003-4550-5128$$aLenz, Oliver$$b15$$eCorresponding author
000578866 773__ $$0PERI:(DE-600)2190276-8$$a10.1038/s41589-022-01226-w$$gVol. 19, no. 4, p. 498 - 506$$n4$$p498 - 506$$tNature chemical biology$$v19$$x1552-4450$$y2023
000578866 8564_ $$uhttps://bib-pubdb1.desy.de/record/578866/files/Post_referee_version.pdf$$yPublished on 2023-01-26. Available in OpenAccess from 2023-07-26.
000578866 8564_ $$uhttps://bib-pubdb1.desy.de/record/578866/files/SI_post_referee_version.pdf$$yRestricted
000578866 8564_ $$uhttps://bib-pubdb1.desy.de/record/578866/files/s41589-022-01226-w.pdf$$yRestricted
000578866 8564_ $$uhttps://bib-pubdb1.desy.de/record/578866/files/Post_referee_version.pdf?subformat=pdfa$$xpdfa$$yPublished on 2023-01-26. Available in OpenAccess from 2023-07-26.
000578866 8564_ $$uhttps://bib-pubdb1.desy.de/record/578866/files/SI_post_referee_version.pdf?subformat=pdfa$$xpdfa$$yRestricted
000578866 8564_ $$uhttps://bib-pubdb1.desy.de/record/578866/files/s41589-022-01226-w.pdf?subformat=pdfa$$xpdfa$$yRestricted
000578866 909CO $$ooai:bib-pubdb1.desy.de:578866$$pdnbdelivery$$pdriver$$pVDB$$popen_access$$popenaire
000578866 9101_ $$0I:(DE-HGF)0$$6P:(DE-H253)PIP1092788$$aTechnische Universität Berlin$$b0
000578866 9101_ $$0I:(DE-HGF)0$$6P:(DE-HGF)0$$a Technische Universität Berlin$$b1
000578866 9101_ $$0I:(DE-HGF)0$$6P:(DE-H253)PIP1085616$$aMax Planck Institute for Chemical Energy Conversion$$b2
000578866 9101_ $$0I:(DE-HGF)0$$6P:(DE-HGF)0$$a Technische Universität Berlin$$b3
000578866 9101_ $$0I:(DE-HGF)0$$6P:(DE-H253)PIP1100547$$aTechnische Universität Berlin$$b4
000578866 9101_ $$0I:(DE-HGF)0$$6P:(DE-HGF)0$$aHumboldt-Universität zu Berlin$$b5
000578866 9101_ $$0I:(DE-HGF)0$$6P:(DE-HGF)0$$aHumboldt-Universität zu Berlin$$b6
000578866 9101_ $$0I:(DE-HGF)0$$6P:(DE-HGF)0$$aTechnische Universität Berlin$$b7
000578866 9101_ $$0I:(DE-588b)2008985-5$$6P:(DE-H253)PIP1016663$$aDeutsches Elektronen-Synchrotron$$b8$$kDESY
000578866 9101_ $$0I:(DE-588)1043621512$$6P:(DE-H253)PIP1016663$$aEuropean XFEL$$b8$$kXFEL.EU
000578866 9101_ $$0I:(DE-HGF)0$$6P:(DE-HGF)0$$aJapan Synchrotron Radiation Research Institute (JASRI)$$b9
000578866 9101_ $$0I:(DE-HGF)0$$6P:(DE-HGF)0$$aTechnische Universität Berlin$$b10
000578866 9101_ $$0I:(DE-HGF)0$$6P:(DE-HGF)0$$a Humboldt-Universität zu Berlin$$b11
000578866 9101_ $$0I:(DE-HGF)0$$6P:(DE-H253)PIP1015325$$aMax Planck Institute for Chemical Energy Conversion$$b12
000578866 9101_ $$0I:(DE-HGF)0$$6P:(DE-H253)PIP1100480$$aTechnische Universität Berlin$$b13
000578866 9101_ $$0I:(DE-HGF)0$$60000-0002-4141-7836$$aTechnische Universität Berlin$$b14
000578866 9101_ $$0I:(DE-HGF)0$$60000-0003-4550-5128$$aTechnische Universität Berlin$$b15
000578866 9131_ $$0G:(DE-HGF)POF4-632$$1G:(DE-HGF)POF4-630$$2G:(DE-HGF)POF4-600$$3G:(DE-HGF)POF4$$4G:(DE-HGF)POF$$aDE-HGF$$bForschungsbereich Materie$$lVon Materie zu Materialien und Leben$$vMaterials – Quantum, Complex and Functional Materials$$x0
000578866 9131_ $$0G:(DE-HGF)POF4-6G3$$1G:(DE-HGF)POF4-6G0$$2G:(DE-HGF)POF4-600$$3G:(DE-HGF)POF4$$4G:(DE-HGF)POF$$aDE-HGF$$bForschungsbereich Materie$$lGroßgeräte: Materie$$vPETRA III (DESY)$$x1
000578866 9141_ $$y2023
000578866 915__ $$0StatID:(DE-HGF)0160$$2StatID$$aDBCoverage$$bEssential Science Indicators$$d2022-11-19
000578866 915__ $$0StatID:(DE-HGF)1190$$2StatID$$aDBCoverage$$bBiological Abstracts$$d2022-11-19
000578866 915__ $$0StatID:(DE-HGF)0113$$2StatID$$aWoS$$bScience Citation Index Expanded$$d2022-11-19
000578866 915__ $$0StatID:(DE-HGF)3003$$2StatID$$aDEAL Nature$$d2022-11-19$$wger
000578866 915__ $$0StatID:(DE-HGF)0530$$2StatID$$aEmbargoed OpenAccess
000578866 915__ $$0StatID:(DE-HGF)0420$$2StatID$$aNationallizenz$$d2023-08-24$$wger
000578866 915__ $$0StatID:(DE-HGF)0200$$2StatID$$aDBCoverage$$bSCOPUS$$d2023-08-24
000578866 915__ $$0StatID:(DE-HGF)0300$$2StatID$$aDBCoverage$$bMedline$$d2023-08-24
000578866 915__ $$0StatID:(DE-HGF)0199$$2StatID$$aDBCoverage$$bClarivate Analytics Master Journal List$$d2023-08-24
000578866 915__ $$0StatID:(DE-HGF)1050$$2StatID$$aDBCoverage$$bBIOSIS Previews$$d2023-08-24
000578866 915__ $$0StatID:(DE-HGF)0150$$2StatID$$aDBCoverage$$bWeb of Science Core Collection$$d2023-08-24
000578866 915__ $$0StatID:(DE-HGF)1030$$2StatID$$aDBCoverage$$bCurrent Contents - Life Sciences$$d2023-08-24
000578866 915__ $$0StatID:(DE-HGF)0100$$2StatID$$aJCR$$bNAT CHEM BIOL : 2022$$d2023-08-24
000578866 915__ $$0StatID:(DE-HGF)0600$$2StatID$$aDBCoverage$$bEbsco Academic Search$$d2023-08-24
000578866 915__ $$0StatID:(DE-HGF)0030$$2StatID$$aPeer Review$$bASC$$d2023-08-24
000578866 915__ $$0StatID:(DE-HGF)9910$$2StatID$$aIF >= 10$$bNAT CHEM BIOL : 2022$$d2023-08-24
000578866 9201_ $$0I:(DE-H253)HAS-User-20120731$$kDOOR ; HAS-User$$lDOOR-User$$x0
000578866 9201_ $$0I:(DE-H253)FS-PET-S-20190712$$kFS-PET-S$$lExperimentebetreuung PETRA III$$x1
000578866 980__ $$ajournal
000578866 980__ $$aVDB
000578866 980__ $$aI:(DE-H253)HAS-User-20120731
000578866 980__ $$aI:(DE-H253)FS-PET-S-20190712
000578866 980__ $$aUNRESTRICTED
000578866 9801_ $$aFullTexts