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@ARTICLE{Mishra:206251,
author = {Mishra, Seema and Stärk, Hans-Joachim and Küpper,
Hendrik},
title = {{A} different sequence of events than previously reported
leads to arsenic-induced damage in {C}eratophyllum demersum
{L}.},
journal = {Metallomics},
volume = {6},
number = {3},
issn = {1756-591X},
address = {Cambridge},
publisher = {RSC Publ.},
reportid = {PUBDB-2015-00765},
pages = {444 - 454},
year = {2014},
abstract = {Arsenic (As) is a common pollutant, and still many
questions remain concerning As toxicity mechanisms under
environmentally relevant conditions in plants. Here we
investigated thresholds and interactions of various proposed
As toxicity mechanisms. Experiments were done under
environmentally pertinent conditions in the rootless aquatic
macrophyte Ceratophyllum demersum L., a model for plant
shoots. Arsenic (provided as As(V)) inhibited plant
metabolism at much lower concentrations and with a different
sequence of events than previously reported. The first
observed effect of toxicity was a decrease in pigment
concentration, it started even at 0.5 μM As. In contrast to
toxic metals, no inhibition of the photosystem II reaction
centre (PSIIRC; measured as Fv/Fm) was found at sublethal As
concentrations. Instead, the decrease in light harvesting
pigments caused a less efficient exciton transfer towards
the PSIIRC. At higher As concentrations this led to
increased non-photochemical quenching (NPQ) by light
harvesting complex II (LHCII). Afterwards, photosynthetic
electron transport decreased, but the increase in starch
content indicated stronger inhibition of starch consumption
than production. At lethal As concentration, photosynthesis
was completely inhibited, its malfunction caused oxidative
stress and not the other way round as reported previously.
Photosynthesis was inhibited before any sign of oxidative
stress was observed. Elevated phosphate drastically shifted
thresholds of lethal As effects, not only by the known
uptake competition but also by modifying uptake regulation
and intracellular processes.},
cin = {DOOR ; HAS-User},
ddc = {690},
cid = {I:(DE-H253)HAS-User-20120731},
pnm = {DORIS Beamline L (POF2-54G13) / PETRA Beamline P06
(POF2-54G14) / FS-Proposal: I-20110682 (I-20110682) /
FS-Proposal: I-20120397 (I-20120397)},
pid = {G:(DE-H253)POF2-L-20130405 / G:(DE-H253)POF2-P06-20130405 /
G:(DE-H253)I-20110682 / G:(DE-H253)I-20120397},
experiment = {EXP:(DE-H253)D-L-20150101 / EXP:(DE-H253)P-P06-20150101},
typ = {PUB:(DE-HGF)16},
UT = {WOS:000333565800006},
pubmed = {pmid:24382492},
doi = {10.1039/c3mt00317e},
url = {https://bib-pubdb1.desy.de/record/206251},
}