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@ARTICLE{Docquier:95357,
author = {Docquier, J. D. and Calderone, V. and De Luca, F. and
Benvenuti, M. and Giuliani, F. and Bellucci, L. and Tafi, A.
and Nordmann, P. and Botta, M. and Rossolini, G. M. and
Mangani, S. and DESY},
title = {{C}rystal structure of the {OXA}-48 beta-lactamase reveals
mechanistic diversity among class {D} carbapenemases.},
journal = {Chemistry $\&$ biology},
volume = {16},
issn = {1074-5521},
address = {Amsterdam [u.a.]},
publisher = {Cell Press},
reportid = {PHPPUBDB-20354},
pages = {540-547},
year = {2009},
note = {(c) Elsevier Ltd; Open Archive},
abstract = {Carbapenem-hydrolyzing class D beta-lactamases (CHDLs) are
enzymes found in important Gram-negative pathogens (mainly
Acinetobacter baumannii and Enterobacteriaceae) that confer
resistance to beta-lactam antibiotics, and notably
carbapenems. The crystal structure of the OXA-48
carbapenemase was determined at pH 7.5 and at a resolution
of 1.9 A. Surprisingly, and by contrast with OXA-24, the
only other CHDL of known crystal structure, the structure of
OXA-48 was similar to OXA-10, an enzyme devoid of
carbapenemase activity, indicating that the hydrolysis of
these compounds could depend on subtle changes in the active
site region. Moreover, the active site groove of OXA-48 was
different from that of OXA-24 in shape, dimensions, and
charge distribution. Molecular dynamics pointed to the
functional relevance of residues located in or close to the
beta5-beta6 loop and allowed us to propose a mechanism for
carbapenem hydrolysis by OXA-48.},
keywords = {Bacterial Proteins: chemistry / Bacterial Proteins:
classification / Catalytic Domain / Computer Simulation /
Crystallography, X-Ray / Kinetics / Protein Structure,
Quaternary / beta-Lactamases: chemistry / beta-Lactamases:
classification / Bacterial Proteins (NLM Chemicals) /
beta-Lactamases (NLM Chemicals) / carbapenemase (NLM
Chemicals)},
cin = {EMBL(-2012)},
ddc = {540},
cid = {$I:(DE-H253)EMBL_-2012_-20130307$},
pnm = {DORIS Beamline BW7 (POF1-550)},
pid = {G:(DE-H253)POF1-BW7-20130405},
experiment = {EXP:(DE-H253)D-BW7-20150101},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:19477418},
UT = {WOS:000266620800009},
doi = {10.1016/j.chembiol.2009.04.010},
url = {https://bib-pubdb1.desy.de/record/95357},
}