% IMPORTANT: The following is UTF-8 encoded. This means that in the presence
% of non-ASCII characters, it will not work with BibTeX 0.99 or older.
% Instead, you should use an up-to-date BibTeX implementation like “bibtex8” or
% “biber”.
@ARTICLE{Haikarainen:95049,
author = {Haikarainen, T. and Paturi, P. and Linden, J. and Haataja,
S. and Meyer-Klaucke, W. and Finne, J. and Papageorgiou, A.
C. and DESY},
title = {{M}agnetic properties and structural characterization of
iron oxide nanoparticles formed by {S}treptococcus suis
{D}pr and four mutants},
journal = {Journal of biological inorganic chemistry},
volume = {16},
issn = {0949-8257},
address = {Berlin},
publisher = {Springer},
reportid = {PHPPUBDB-19320},
pages = {799-807},
year = {2011},
abstract = {Streptococcus suis Dpr belongs to the Dps family of
bacterial and archaeal proteins that oxidize Fe(2+) to
Fe(3+) to protect microorganisms from oxidative damage. The
oxidized iron is subsequently deposited as ferrihydrite
inside a protein cavity, resulting in the formation of an
iron core. The size and the magnetic properties of the iron
core have attracted considerable attention for
nanotechnological applications in recent years. Here, the
magnetic and structural properties of the iron core in
wild-type Dpr and four cavity mutants were studied. All
samples clearly demonstrated a superparamagnetic behavior in
superconducting quantum interference device magnetometry and
Mössbauer spectroscopy compatible with that of
superparamagnetic ferrihydrite nanoparticles. However, all
the mutants exhibited higher magnetic moments than the
wild-type protein. Furthermore, measurement of the iron
content with inductively coupled plasma mass spectrometry
revealed a smaller amount of iron in the iron cores of the
mutants, suggesting that the mutations affect nucleation and
iron deposition inside the cavity. The X-ray crystal
structures of the mutants revealed no changes compared with
the wild-type crystal structure; thus, the differences in
the magnetic moments could not be attributed to structural
changes in the protein. Extended X-ray absorption fine
structure measurements showed that the coordination geometry
of the iron cores of the mutants was similar to that of the
wild-type protein. Taken together, these results suggest
that mutation of the residues that surround the iron storage
cavity could be exploited to selectively modify the magnetic
properties of the iron core without affecting the structure
of the protein and the geometry of the iron core.},
keywords = {Bacterial Proteins: chemistry / Bacterial Proteins:
genetics / Bacterial Proteins: metabolism / Ceruloplasmin:
metabolism / Crystallography, X-Ray / Ferric Compounds:
chemistry / Ferric Compounds: metabolism / Ferritins:
metabolism / Magnetics / Nanoparticles: chemistry / Point
Mutation / Protein Engineering / Spectroscopy, Mossbauer /
Streptococcus suis: chemistry / Streptococcus suis: genetics
/ Streptococcus suis: metabolism / Bacterial Proteins (NLM
Chemicals) / Ferric Compounds (NLM Chemicals) / ferric oxide
(NLM Chemicals) / Ferritins (NLM Chemicals) / Ceruloplasmin
(NLM Chemicals)},
cin = {EMBL},
ddc = {540},
cid = {$I:(DE-H253)EMBL_-2012_-20130307$},
pnm = {DORIS Beamline K1.2 (POF2-54G13)},
pid = {G:(DE-H253)POF2-K1.2-20130405},
experiment = {EXP:(DE-H253)D-K1.2-20150101},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:21487937},
UT = {WOS:000290773500012},
doi = {10.1007/s00775-011-0781-z},
url = {https://bib-pubdb1.desy.de/record/95049},
}