TY  - JOUR
AU  - Cao, X.
AU  - Coskun, Ü.
AU  - Rössle, M.
AU  - Buschhorn, S. B.
AU  - Grzybek, M.
AU  - Dafforn, T. R.
AU  - Lenoir, M.
AU  - Overduin, M.
AU  - Simons, K.
AU  - DESY
TI  - Golgi protein FAPP2 tubulates membranes
JO  - Proceedings of the National Academy of Sciences of the United States of America
VL  - 106
SN  - 1091-6490
CY  - Washington, DC
PB  - Academy
M1  - PHPPUBDB-12319
SP  - 21121-21125
PY  - 2009
AB  - The Golgi-associated four-phosphate adaptor protein 2 (FAPP2) has been shown to possess transfer activity for glucosylceramide both in vitro and in cells. We have previously shown that FAPP2 is involved in apical transport from the Golgi complex in epithelial MDCK cells. In this paper we assign an unknown activity for the protein as well as providing structural insight into protein assembly and a low-resolution envelope structure. By applying analytical ultracentrifugation and small-angle x-ray scattering, we show that FAPP2 is a dimeric protein in solution, having a curved shape 30 nm in length. The purified FAPP2 protein has the capability to form tubules from membrane sheets in vitro. This activity is dependent on the phosphoinositide-binding activity of the PH domain of FAPP2. These data suggest that FAPP2 functions directly in the formation of apical carriers in the trans-Golgi network.
KW  - Adaptor Proteins, Signal Transducing: chemistry
KW  - Adaptor Proteins, Signal Transducing: metabolism
KW  - Adaptor Proteins, Signal Transducing: physiology
KW  - Animals
KW  - Cell Line
KW  - Dogs
KW  - Lipid Bilayers: metabolism
KW  - Phosphatidylinositols: metabolism
KW  - Protein Binding
KW  - Protein Conformation
KW  - Protein Multimerization
KW  - Solutions
KW  - trans-Golgi Network
KW  - Adaptor Proteins, Signal Transducing (NLM Chemicals)
KW  - Lipid Bilayers (NLM Chemicals)
KW  - PLEKHA8 protein, human (NLM Chemicals)
KW  - Phosphatidylinositols (NLM Chemicals)
KW  - Solutions (NLM Chemicals)
LB  - PUB:(DE-HGF)16
C6  - pmid:19940249
C2  - pmc:PMC2795549
UR  - <Go to ISI:>//WOS:000272795300022
DO  - DOI:10.1073/pnas.0911789106
UR  - https://bib-pubdb1.desy.de/record/94235
ER  -