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@ARTICLE{Fedorov:93921,
author = {Fedorov, Roman and Böhl, Markus and Tsiavaliaris, Georgios
and Hartmann, Falk K and Taft, Manuel H and Baruch, Petra
and Brenner, Bernhard and Martin, René and Knölker,
Hans-Joachim and Gutzeit, Herwig O and Manstein, Dietmar J
and DESY},
title = {{T}he mechanism of pentabromopseudilin inhibition of myosin
motor activity},
journal = {Nature structural $\&$ molecular biology},
volume = {16},
number = {1},
issn = {1072-8368},
address = {London [u.a.]},
publisher = {Nature Publishing Group},
reportid = {PHPPUBDB-11987},
pages = {80 - 88},
year = {2009},
note = {©Nature America, INC},
abstract = {We have identified pentabromopseudilin (PBP) as a potent
inhibitor of myosin-dependent processes such as isometric
tension development and unloaded shortening velocity.
PBP-induced reductions in the rate constants for ATP
binding, ATP hydrolysis and ADP dissociation extend the time
required per myosin ATPase cycle in the absence and presence
of actin. Additionally, coupling between the actin and
nucleotide binding sites is reduced in the presence of the
inhibitor. The selectivity of PBP differs from that observed
with other myosin inhibitors. To elucidate the binding mode
of PBP, we crystallized the Dictyostelium myosin-2 motor
domain in the presence of Mg2+-ADP–meta-vanadate and PBP.
The electron density for PBP is unambiguous and shows PBP to
bind at a previously unknown allosteric site near the tip of
the 50-kDa domain, at a distance of 16 Å from the
nucleotide binding site and 7.5 Å away from the
blebbistatin binding pocket.},
cin = {EMBL(-2012)},
ddc = {570},
cid = {$I:(DE-H253)EMBL_-2012_-20130307$},
pnm = {FS Beamline without reference (POF1-550)},
pid = {G:(DE-H253)POF1-No-Ref-20130405},
experiment = {EXP:(DE-H253)Unknown-BL-20150101},
typ = {PUB:(DE-HGF)16},
UT = {WOS:00},
pubmed = {pmid:19122661},
doi = {10.1038/nsmb.1542},
url = {https://bib-pubdb1.desy.de/record/93921},
}