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@ARTICLE{Seibert:92559,
      author       = {Seibert, M. M. and Ekeberg, T. and Maia, F. R. N. C. and
                      Svenda, M. and Andreasson, J. and Jonsson, O. and Odic, D.
                      and Iwan, B. and Rocker, A. and Westphal, D. and Hantke, M.
                      and DePonte, D. P. and Barty, A. and Schulz, J. and
                      Gumprecht, L. and Coppola, N. and Aquila, A. and Liang, M.
                      and White, T. A. and Martin, A. and Caleman, C. and Stern,
                      S. and Abergel, C. and Seltzer, V. and Claverie, J.-M. and
                      Bostedt, C. and Bozek, J. D. and Boutet, S. and Miahnahri,
                      A. A. and Messerschmidt, M. and Krzywinski, J. and Williams,
                      G. and Hodgson, K. O. and Bogan, M. J. and Hampton, C. Y.
                      and Sierra, R. G. and Starodub, D. and Andersson, I. and
                      Bajt, S. and Barthelmess, M. and Spence, J. C. H. and
                      Fromme, P. and Weierstall, U. and Kirian, R. and Hunter, M.
                      and Doak, R. B. and Marchesini, S. and Hau-Riege, S. P. and
                      Frank, M. and Shoeman, R. L. and Lomb, L. and Epp, S. W. and
                      Hartmann, R. and Rolles, D. and Rudenko, A. and Schmidt, C.
                      and Foucar, L. and Kimmel, N. and Holl, P. and Rudek, B. and
                      Erk, B. and Homke, A. and Reich, C. and Pietschner, D. and
                      Weidenspointner, G. and Struder, L. and Hauser, G. and
                      Gorke, H. and Ullrich, J. and Schlichting, I. and Herrmann,
                      S. and Schaller, G. and Schopper, F. and Soltau, H. and
                      Kühnel, K.-U. and Andritschke, R. and Schröter, C.-D. and
                      Krasniqi, F. and Bott, M. and Schorb, S. and Rupp, D. and
                      Adolph, M. and Gorkhover, T. and Hirsemann, H. and Potdevin,
                      G. and Graafsma, H. and Nilsson, B. and Chapman, H. N. and
                      Hajdu, J. and DESY},
      title        = {{S}ingle mimivirus particles intercepted and imaged with an
                      x-ray laser},
      journal      = {Nature},
      volume       = {470},
      issn         = {0028-0836},
      address      = {London [u.a.]},
      publisher    = {Nature Publising Group},
      reportid     = {PHPPUBDB-16205},
      pages        = {78-81},
      year         = {2011},
      abstract     = {X-ray lasers offer new capabilities in understanding the
                      structure of biological systems, complex materials and
                      matter under extreme conditions. Very short and extremely
                      bright, coherent X-ray pulses can be used to outrun key
                      damage processes and obtain a single diffraction pattern
                      from a large macromolecule, a virus or a cell before the
                      sample explodes and turns into plasma. The continuous
                      diffraction pattern of non-crystalline objects permits
                      oversampling and direct phase retrieval. Here we show that
                      high-quality diffraction data can be obtained with a single
                      X-ray pulse from a non-crystalline biological sample, a
                      single mimivirus particle, which was injected into the
                      pulsed beam of a hard-X-ray free-electron laser, the Linac
                      Coherent Light Source. Calculations indicate that the energy
                      deposited into the virus by the pulse heated the particle to
                      over 100,000 K after the pulse had left the sample. The
                      reconstructed exit wavefront (image) yielded 32-nm
                      full-period resolution in a single exposure and showed no
                      measurable damage. The reconstruction indicates
                      inhomogeneous arrangement of dense material inside the
                      virion. We expect that significantly higher resolutions will
                      be achieved in such experiments with shorter and brighter
                      photon pulses focused to a smaller area. The resolution in
                      such experiments can be further extended for samples
                      available in multiple identical copies.},
      keywords     = {Electrons / Hot Temperature / Lasers / Mimiviridae:
                      chemistry / Photons / Time Factors / X-Ray Diffraction:
                      instrumentation / X-Ray Diffraction: methods / X-Rays},
      cin          = {HASYLAB(-2012) / FS-CFEL-1 / MPG / MPSD / Eur.XFEL},
      ddc          = {070},
      cid          = {$I:(DE-H253)HASYLAB_-2012_-20130307$ /
                      I:(DE-H253)FS-CFEL-1-20120731 / I:(DE-H253)MPG-20120806 /
                      I:(DE-H253)MPSD-20120731 / $I:(DE-H253)Eur_XFEL-20120731$},
      pnm          = {Experiments at CFEL (POF2-544)},
      pid          = {G:(DE-H253)POF2-CFEL-Exp.-20130405},
      experiment   = {EXP:(DE-H253)CFEL-Exp-20150101},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:21293374},
      UT           = {WOS:000286886400037},
      doi          = {10.1038/nature09748},
      url          = {https://bib-pubdb1.desy.de/record/92559},
}