Home > Publications database > The Crystal Structure of C176A Mutated [Fe]-Hydrogenase Suggests an Acyl-Iron Ligation in the Active Site Iron Complex > print

001     87264
005     20250731122201.0
024 7 _ |2 pmid
|a pmid:19162018
024 7 _ |2 ISSN
|a 1873-3468
024 7 _ |2 ISSN
|a 0014-5793
024 7 _ |2 WOS
|a WOS:000263603600014
024 7 _ |2 doi
|a 10.1016/j.febslet.2009.01.017
024 7 _ |2 altmetric
|a altmetric:520977
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|2 openalex
037 _ _ |a PHPPUBDB-8575
041 _ _ |a eng
082 _ _ |a 570
100 1 _ |0 P:(DE-HGF)0
|a Hiromoto, Takashi
|b 0
110 1 _ |a DESY
|b Experiments with synchrotron radiation
245 _ _ |a The Crystal Structure of C176A Mutated [Fe]-Hydrogenase Suggests an Acyl-Iron Ligation in the Active Site Iron Complex
260 _ _ |a Amsterdam [u.a.]
|b Elsevier
|c 2009
300 _ _ |a 585-590
336 7 _ |2 DRIVER
|a article
336 7 _ |2 DataCite
|a Output Types/Journal article
336 7 _ |0 PUB:(DE-HGF)16
|2 PUB:(DE-HGF)
|a Journal Article
|b journal
|m journal
|s 1481730256_13084
336 7 _ |2 BibTeX
|a ARTICLE
336 7 _ |2 ORCID
|a JOURNAL_ARTICLE
336 7 _ |0 0
|2 EndNote
|a Journal Article
440 _ 0 |0 PERI:(DE-600)1460391-3
|a FEBS Letters
|v 583
|x 0014-5793
|y 3
500 _ _ |3 Converted on 2013-05-30 12:56
|a (c) Federation of European Biochemical Societies. Post referee full text in progress (embargo 1 year from 20 JAN 2009).
500 _ _ |3 Converted on 2013-06-21 19:20
520 _ _ |a [Fe]-hydrogenase is one of three types of enzymes known to activate H(2). Crystal structure analysis recently revealed that its active site iron is ligated square-pyramidally by Cys176-sulfur, two CO, an "unknown" ligand and the sp(2)-hybridized nitrogen of a unique iron-guanylylpyridinol-cofactor. We report here on the structure of the C176A mutated enzyme crystallized in the presence of dithiothreitol (DTT). It suggests an iron center octahedrally coordinated by one DTT-sulfur and one DTT-oxygen, two CO, the 2-pyridinol's nitrogen and the 2-pyridinol's 6-formylmethyl group in an acyl-iron ligation. This result led to a re-interpretation of the iron ligation in the wild-type.
536 _ _ |0 G:(DE-H253)POF1-No-Ref-20130405
|f POF I
|x 0
|c POF1-550
|a FS Beamline without reference (POF1-550)
588 _ _ |a Dataset connected to Pubmed
650 _ 7 |0 0
|2 NLM Chemicals
|a Holoenzymes
650 _ 7 |0 0
|2 NLM Chemicals
|a Iron-Sulfur Proteins
650 _ 7 |0 71-30-7
|2 NLM Chemicals
|a Cytosine
650 _ 7 |0 73-24-5
|2 NLM Chemicals
|a Adenine
650 _ 7 |0 7439-89-6
|2 NLM Chemicals
|a Iron
650 _ 7 |0 EC 1.12.-
|2 NLM Chemicals
|a iron hydrogenase
650 _ 7 |0 EC 1.12.7.2
|2 NLM Chemicals
|a Hydrogenase
650 _ 2 |2 MeSH
|a Adenine: metabolism
650 _ 2 |2 MeSH
|a Catalytic Domain
650 _ 2 |2 MeSH
|a Crystallography, X-Ray
650 _ 2 |2 MeSH
|a Cytosine: metabolism
650 _ 2 |2 MeSH
|a Holoenzymes: chemistry
650 _ 2 |2 MeSH
|a Holoenzymes: genetics
650 _ 2 |2 MeSH
|a Holoenzymes: metabolism
650 _ 2 |2 MeSH
|a Hydrogenase: chemistry
650 _ 2 |2 MeSH
|a Hydrogenase: genetics
650 _ 2 |2 MeSH
|a Hydrogenase: metabolism
650 _ 2 |2 MeSH
|a Iron: chemistry
650 _ 2 |2 MeSH
|a Iron: metabolism
650 _ 2 |2 MeSH
|a Iron-Sulfur Proteins: chemistry
650 _ 2 |2 MeSH
|a Iron-Sulfur Proteins: genetics
650 _ 2 |2 MeSH
|a Iron-Sulfur Proteins: metabolism
650 _ 2 |2 MeSH
|a Methanococcales: enzymology
650 _ 2 |2 MeSH
|a Methanococcales: genetics
650 _ 2 |2 MeSH
|a Mutation: genetics
650 _ 2 |2 MeSH
|a Protein Structure, Quaternary
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|6 EXP:(DE-H253)Unknown-BL-20150101
|f Unknown DESY Beamline
|x 0
700 1 _ |0 P:(DE-HGF)0
|a Ataka, Kenichi
|b 1
700 1 _ |0 P:(DE-HGF)0
|a Pilak, Oliver
|b 2
700 1 _ |a Vogt, S.
|b 3
700 1 _ |0 P:(DE-HGF)0
|a Salamone Stagni, Marco
|b 4
700 1 _ |0 P:(DE-H253)PIP1001970
|a Meyer-Klaucke, W.
|b 5
700 1 _ |0 P:(DE-HGF)0
|a Warkentin, Eberhard
|b 6
700 1 _ |0 P:(DE-HGF)0
|a Thauer, Rudolf K.
|b 7
700 1 _ |0 P:(DE-HGF)0
|a Shima, Seigo
|b 8
|e Corresponding author
700 1 _ |0 P:(DE-H253)PIP1018667
|a Ermler, U.
|b 9
|e Corresponding author
773 _ _ |0 PERI:(DE-600)1460391-3
|a 10.1016/j.febslet.2009.01.017
|g Vol. 583, p. 585-590
|p 585-590
|q 583<585-590
|t FEBS letters
|v 583
|x 0014-5793
|y 2009
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913 1 _ |0 G:(DE-HGF)POF1-550
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|v Großgeräte für die Forschung mit Photonen, Neutronen, Ionen
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|b Struktur der Materie
|l Großgeräte für die Forschung mit Photonen, Neutronen, Ionen
914 1 _ |a (c) Elsevier. No copyright permission for full text.
|y 2009
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920 _ _ |k 001
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