TY  - JOUR
AU  - Hiromoto, Takashi
AU  - Ataka, Kenichi
AU  - Pilak, Oliver
AU  - Vogt, S.
AU  - Salamone Stagni, Marco
AU  - Meyer-Klaucke, W.
AU  - Warkentin, Eberhard
AU  - Thauer, Rudolf K.
AU  - Shima, Seigo
AU  - Ermler, U.
AU  - DESY
TI  - The Crystal Structure of C176A Mutated [Fe]-Hydrogenase Suggests an Acyl-Iron Ligation in the Active Site Iron Complex
JO  - FEBS letters
VL  - 583
SN  - 0014-5793
CY  - Amsterdam [u.a.]
PB  - Elsevier
M1  - PHPPUBDB-8575
SP  - 585-590
PY  - 2009
N1  - (c) Federation of European Biochemical Societies. Post referee full text in progress (embargo 1 year from 20 JAN 2009).
AB  - [Fe]-hydrogenase is one of three types of enzymes known to activate H(2). Crystal structure analysis recently revealed that its active site iron is ligated square-pyramidally by Cys176-sulfur, two CO, an ünknown" ligand and the sp(2)-hybridized nitrogen of a unique iron-guanylylpyridinol-cofactor. We report here on the structure of the C176A mutated enzyme crystallized in the presence of dithiothreitol (DTT). It suggests an iron center octahedrally coordinated by one DTT-sulfur and one DTT-oxygen, two CO, the 2-pyridinol's nitrogen and the 2-pyridinol's 6-formylmethyl group in an acyl-iron ligation. This result led to a re-interpretation of the iron ligation in the wild-type.
KW  - Adenine: metabolism
KW  - Catalytic Domain
KW  - Crystallography, X-Ray
KW  - Cytosine: metabolism
KW  - Holoenzymes: chemistry
KW  - Holoenzymes: genetics
KW  - Holoenzymes: metabolism
KW  - Hydrogenase: chemistry
KW  - Hydrogenase: genetics
KW  - Hydrogenase: metabolism
KW  - Iron: chemistry
KW  - Iron: metabolism
KW  - Iron-Sulfur Proteins: chemistry
KW  - Iron-Sulfur Proteins: genetics
KW  - Iron-Sulfur Proteins: metabolism
KW  - Methanococcales: enzymology
KW  - Methanococcales: genetics
KW  - Mutation: genetics
KW  - Protein Structure, Quaternary
KW  - Holoenzymes (NLM Chemicals)
KW  - Iron-Sulfur Proteins (NLM Chemicals)
KW  - Cytosine (NLM Chemicals)
KW  - Adenine (NLM Chemicals)
KW  - Iron (NLM Chemicals)
KW  - iron hydrogenase (NLM Chemicals)
KW  - Hydrogenase (NLM Chemicals)
LB  - PUB:(DE-HGF)16
C6  - pmid:19162018
UR  - <Go to ISI:>//WOS:000263603600014
DO  - DOI:10.1016/j.febslet.2009.01.017
UR  - https://bib-pubdb1.desy.de/record/87264
ER  -