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@ARTICLE{Havukainen:85833,
author = {Havukainen, H. and Haataja, S. and Kauko, A. and
Pulliainen, A. T. and Salminen, A. and Haikarainen, T. and
Finne, J. and Papageorgiou, A. C. and DESY},
title = {{S}tructural basis of zinc- and terbium-mediated inhibition
of ferroxidase activity in {D}ps ferritin-like proteins},
journal = {Protein science},
volume = {17},
issn = {0961-8368},
address = {Hoboken, NJ},
publisher = {Wiley},
reportid = {PHPPUBDB-8588},
pages = {1513-1521},
year = {2008},
abstract = {Streptococcus suis Dpr is an iron-binding protein involved
in oxidative stress resistance. It belongs to the bacterial
Dps protein family whose members form dodecameric
assemblies. Previous studies have shown that zinc and
terbium inhibit iron incorporation in Listeria innocua Dps
protein. In order to gain structural insights into the
inhibitory effect of zinc and terbium, the crystal
structures of Streptococcus suis Dpr complexes with these
ions were determined at 1.8 A and 2.1 A, respectively. Both
ions were found to bind at the ferroxidase center and in the
same location as iron. In addition, a novel zinc-binding
site formed by His40 and His44 was identified. Both His
residues were found to be present within all known
Streptococcus suis Dpr variants and in Streptococcus
pneumoniae, Streptococcus gordonii, and Streptococcus
sanguinis Dpr proteins. Amino acid sequence alignment of Dpr
with other Dps family members revealed that His44 is highly
conserved, in contrast to His40. The inhibitory effect of
zinc and terbium on iron oxidation in Dpr was studied in
vitro, and it was found that both ions at concentrations
>0.2 mM almost completely abolish iron binding. These
results provide a structural basis for the inhibitory effect
of zinc and terbium in the Dps family of proteins, and
suggest a potential role of the Dps proteins in zinc
detoxification mechanisms involving the second zinc-binding
site.},
keywords = {Amino Acid Sequence / Bacterial Proteins: chemistry /
Bacterial Proteins: genetics / Bacterial Proteins:
metabolism / Binding Sites / Ceruloplasmin: antagonists $\&$
inhibitors / Crystallography, X-Ray / DNA-Binding Proteins:
chemistry / DNA-Binding Proteins: genetics / DNA-Binding
Proteins: metabolism / Ferritins: metabolism / Genes,
Bacterial / Histidine: chemistry / Humans / Models,
Molecular / Molecular Sequence Data / Protein Binding /
Sequence Homology, Amino Acid / Streptococcus suis:
chemistry / Streptococcus suis: genetics / Streptococcus
suis: isolation $\&$ purification / Streptococcus suis:
metabolism / Terbium: chemistry / Terbium: metabolism /
Water: chemistry / Zinc: chemistry / Zinc: metabolism /
Bacterial Proteins (NLM Chemicals) / DNA-Binding Proteins
(NLM Chemicals) / DPS protein, Bacteria (NLM Chemicals) /
Histidine (NLM Chemicals) / Terbium (NLM Chemicals) / Zinc
(NLM Chemicals) / Water (NLM Chemicals) / Ferritins (NLM
Chemicals) / Ceruloplasmin (NLM Chemicals)},
cin = {EMBL},
ddc = {610},
cid = {$I:(DE-H253)EMBL_-2012_-20130307$},
pnm = {FS Beamline without reference (POF1-550)},
pid = {G:(DE-H253)POF1-No-Ref-20130405},
experiment = {EXP:(DE-H253)Unknown-BL-20150101},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:18552126},
pmc = {pmc:PMC2525527},
UT = {WOS:000258651900006},
doi = {10.1110/ps.036236.108},
url = {https://bib-pubdb1.desy.de/record/85833},
}