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@ARTICLE{Bulska:81075,
author = {Bulska, E. and Wysocka, I. A. and Wierzbicka, M. H. and
Proost, K. and Janssens, K. and Falkenberg, G. and DESY},
title = {{I}n vivo investigation of the distribution and the local
speciation of selenium in {A}llium cepa {L}. by means of
microscopic {X}-ray absorption near-edge structure
spectroscopy and confocal microscopic {X}-ray fluorescence
analysis},
journal = {Analytical chemistry},
volume = {78},
issn = {0003-2700},
address = {Columbus, Ohio},
publisher = {American Chemical Society},
reportid = {PHPPUBDB-3402},
pages = {7616-7624},
year = {2006},
abstract = {In this work, microscopic X-ray absorption near-edge
structure spectroscopy (mu-XANES) and confocal microscopic
X-ray fluorescence analysis (mu-XRF) were used for the in
vivo determination of the distribution of total selenium and
for the local speciation of selenium in roots and leaves of
onion. Selected Allium cepa L. plants were grown
hydroponically in a standard medium containing inorganic
selenium compounds (selenite or selenate). The measurements
were performed in vivo, that is, on living plants without
the need for any form of sampling or sample pretreatment and
without the necessity for cutting plant tissues into pieces.
Distinct energy differences of the XANES spectra of various
selenium reference compounds having different oxidation
states allow adjusting the excitation energies used for
mu-XRF mapping in such a manner that the distribution of
selenium in various oxidation states is obtained with a
spatial resolution of a few tens of micrometers within the
virtual cross section of the onion tissues. We find that the
ratio of inorganic to amino acid selenium compounds differs
in various subparts of the plant. Detailed in vivo
investigation of the distribution of various selenium
species in virtual cross sections of root tips and green
leaf shows that the selenium transport takes place via
different mechanisms, depending on the nature of the
selenium compounds originally taken up.},
keywords = {Amino Acids: analysis / Microscopy, Confocal: methods /
Microscopy, Fluorescence: methods / Onions: chemistry /
Organoselenium Compounds: analysis / Oxidation-Reduction /
Plant Leaves: chemistry / Plant Leaves: ultrastructure /
Plant Roots: chemistry / Plant Roots: ultrastructure /
Selenium: analysis / Spectrometry, X-Ray Emission: methods /
Amino Acids (NLM Chemicals) / Organoselenium Compounds (NLM
Chemicals) / Selenium (NLM Chemicals)},
cin = {HASYLAB},
ddc = {540},
cid = {$I:(DE-H253)HASYLAB_-2012_-20130307$},
pnm = {DORIS Beamline L (POF1-550)},
pid = {G:(DE-H253)POF1-L-20130405},
experiment = {EXP:(DE-H253)D-L-20150101},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:17105151},
UT = {WOS:000242021400003},
doi = {10.1021/ac060380s},
url = {https://bib-pubdb1.desy.de/record/81075},
}