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000081075 0247_ $$2doi$$a10.1021/ac060380s
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000081075 041__ $$aeng
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000081075 1001_ $$aBulska, E.
000081075 1101_ $$aDESY$$bExperiments with synchrotron radiation
000081075 245__ $$aIn vivo investigation of the distribution and the local speciation of selenium in Allium cepa L. by means of microscopic X-ray absorption near-edge structure spectroscopy and confocal microscopic X-ray fluorescence analysis
000081075 260__ $$aColumbus, Ohio$$bAmerican Chemical Society$$c2006
000081075 300__ $$a7616-7624
000081075 3367_ $$00$$2EndNote$$aJournal Article
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000081075 440_0 $$0PERI:(DE-600)1483443-1$$aAnal. Chem.$$v78$$x0003-2700$$y22
000081075 500__ $$3Converted on 2013-05-30 11:18
000081075 500__ $$3Converted on 2013-06-21 19:20
000081075 520__ $$aIn this work, microscopic X-ray absorption near-edge structure spectroscopy (mu-XANES) and confocal microscopic X-ray fluorescence analysis (mu-XRF) were used for the in vivo determination of the distribution of total selenium and for the local speciation of selenium in roots and leaves of onion. Selected Allium cepa L. plants were grown hydroponically in a standard medium containing inorganic selenium compounds (selenite or selenate). The measurements were performed in vivo, that is, on living plants without the need for any form of sampling or sample pretreatment and without the necessity for cutting plant tissues into pieces. Distinct energy differences of the XANES spectra of various selenium reference compounds having different oxidation states allow adjusting the excitation energies used for mu-XRF mapping in such a manner that the distribution of selenium in various oxidation states is obtained with a spatial resolution of a few tens of micrometers within the virtual cross section of the onion tissues. We find that the ratio of inorganic to amino acid selenium compounds differs in various subparts of the plant. Detailed in vivo investigation of the distribution of various selenium species in virtual cross sections of root tips and green leaf shows that the selenium transport takes place via different mechanisms, depending on the nature of the selenium compounds originally taken up.
000081075 536__ $$0G:(DE-H253)POF1-L-20130405$$aDORIS Beamline L (POF1-550)$$cPOF1-550$$fPOF I$$x0
000081075 588__ $$aDataset connected to Pubmed
000081075 650_2 $$2MeSH$$aAmino Acids: analysis
000081075 650_2 $$2MeSH$$aMicroscopy, Confocal: methods
000081075 650_2 $$2MeSH$$aMicroscopy, Fluorescence: methods
000081075 650_2 $$2MeSH$$aOnions: chemistry
000081075 650_2 $$2MeSH$$aOrganoselenium Compounds: analysis
000081075 650_2 $$2MeSH$$aOxidation-Reduction
000081075 650_2 $$2MeSH$$aPlant Leaves: chemistry
000081075 650_2 $$2MeSH$$aPlant Leaves: ultrastructure
000081075 650_2 $$2MeSH$$aPlant Roots: chemistry
000081075 650_2 $$2MeSH$$aPlant Roots: ultrastructure
000081075 650_2 $$2MeSH$$aSelenium: analysis
000081075 650_2 $$2MeSH$$aSpectrometry, X-Ray Emission: methods
000081075 650_7 $$00$$2NLM Chemicals$$aAmino Acids
000081075 650_7 $$00$$2NLM Chemicals$$aOrganoselenium Compounds
000081075 650_7 $$07782-49-2$$2NLM Chemicals$$aSelenium
000081075 693__ $$0EXP:(DE-H253)D-L-20150101$$1EXP:(DE-H253)DORISIII-20150101$$6EXP:(DE-H253)D-L-20150101$$aDORIS III$$fDORIS Beamline L$$x0
000081075 7001_ $$aWysocka, I. A.
000081075 7001_ $$aWierzbicka, M. H.
000081075 7001_ $$aProost, K.
000081075 7001_ $$aJanssens, K.
000081075 7001_ $$aFalkenberg, G.
000081075 773__ $$0PERI:(DE-600)1483443-1$$a10.1021/ac060380s$$gVol. 78, p. 7616-7624$$p7616-7624$$q78<7616-7624$$tAnalytical chemistry$$v78$$x0003-2700$$y2006
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000081075 9101_ $$0I:(DE-588b)2008985-5$$aDeutsches Elektronen-Synchrotron$$kDESY
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000081075 9141_ $$aNo copyright permission for full text$$y2006
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000081075 920_1 $$iExperiments with synchrotron radiation$$kHASYLAB
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