TY  - JOUR
AU  - Zou, P.
AU  - Pinotsis, N.
AU  - Lange, S.
AU  - Song, Y. H.
AU  - Popov, A.
AU  - Mavridis, I.
AU  - Mayans, O. M.
AU  - Gautel, M.
AU  - Wilmanns, M.
AU  - DESY
TI  - Palindromic assembly of the giant muscle protein titin in the sarcomeric Z-disk
JO  - Nature 
VL  - 439
SN  - 0028-0836
CY  - London [u.a.]
PB  - Nature Publising Group
M1  - PHPPUBDB-3070
SP  - 233
PY  - 2006
AB  - The Z-disk of striated and cardiac muscle sarcomeres is one of the most densely packed cellular structures in eukaryotic cells. It provides the architectural framework for assembling and anchoring the largest known muscle filament systems by an extensive network of protein-protein interactions, requiring an extraordinary level of mechanical stability. Here we show, using X-ray crystallography, how the amino terminus of the longest filament component, the giant muscle protein titin, is assembled into an antiparallel (2:1) sandwich complex by the Z-disk ligand telethonin. The pseudosymmetric structure of telethonin mediates a unique palindromic arrangement of two titin filaments, a type of molecular assembly previously found only in protein-DNA complexes. We have confirmed its unique architecture in vivo by protein complementation assays, and in vitro by experiments using fluorescence resonance energy transfer. The model proposed may provide a molecular paradigm of how major sarcomeric filaments are crosslinked, anchored and aligned within complex cytoskeletal networks.
KW  - Amino Acid Sequence
KW  - Animals
KW  - Animals, Newborn
KW  - Crystallography, X-Ray
KW  - Fluorescence Resonance Energy Transfer
KW  - Genetic Complementation Test
KW  - Hydrogen Bonding
KW  - Ligands
KW  - Models, Molecular
KW  - Molecular Sequence Data
KW  - Multiprotein Complexes: chemistry
KW  - Multiprotein Complexes: metabolism
KW  - Muscle Proteins: chemistry
KW  - Muscle Proteins: genetics
KW  - Muscle Proteins: metabolism
KW  - Myocytes, Cardiac: chemistry
KW  - Myocytes, Cardiac: metabolism
KW  - Protein Binding
KW  - Protein Conformation
KW  - Protein Kinases: chemistry
KW  - Protein Kinases: genetics
KW  - Protein Kinases: metabolism
KW  - Rats
KW  - Sarcomeres: chemistry
KW  - Sarcomeres: metabolism
KW  - Ligands (NLM Chemicals)
KW  - Multiprotein Complexes (NLM Chemicals)
KW  - Muscle Proteins (NLM Chemicals)
KW  - connectin (NLM Chemicals)
KW  - telethonin protein, rat (NLM Chemicals)
KW  - Protein Kinases (NLM Chemicals)
LB  - PUB:(DE-HGF)16
C6  - pmid:16407954
UR  - <Go to ISI:>//WOS:000234538400046
DO  - DOI:10.1038/nature04343
UR  - https://bib-pubdb1.desy.de/record/80904
ER  -