000646263 001__ 646263
000646263 005__ 20260220211036.0
000646263 0247_ $$2doi$$a10.1042/BCJ20253442
000646263 0247_ $$2ISSN$$a1470-8728
000646263 0247_ $$2ISSN$$a0264-6021
000646263 0247_ $$2datacite_doi$$a10.3204/PUBDB-2026-00786
000646263 037__ $$aPUBDB-2026-00786
000646263 041__ $$aEnglish
000646263 082__ $$a540
000646263 1001_ $$0P:(DE-H253)PIP1121049$$aDorendorf, Till$$b0
000646263 245__ $$aMolecular identifiers of the evolutionarily conserved titin pseudokinase
000646263 260__ $$aLondon [u.a.]$$bPortland Press$$c2026
000646263 3367_ $$2DRIVER$$aarticle
000646263 3367_ $$2DataCite$$aOutput Types/Journal article
000646263 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article$$bjournal$$mjournal$$s1771590551_4115155
000646263 3367_ $$2BibTeX$$aARTICLE
000646263 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000646263 3367_ $$00$$2EndNote$$aJournal Article
000646263 500__ $$aISSN 1470-8728 not unique: **2 hits**.
000646263 520__ $$aTitin kinase (TK) is an enigmatic pseudokinase specific to the striated muscle of vertebrates. Embedded within the contractile sarcomere and flanked by extensible regulatory tails, TK is thought to act as a mechanoreceptor that senses mechanical signals arising from muscle function. Studies on TK so far have focused narrowly on the human representative, whose phosphotransfer activity remains questioned. To investigate whether the pseudokinase character is a hallmark of TK, we studied sequences of distantly evolved fish representatives and rationalized conservation patterns by solving the crystal structure of TK from medaka (isoform b). We find that sequence deviations in functional motifs involved in ATP and magnesium binding, respectively θxK (θ: bulky hydrophobic residue) and EFG, are evolutionarily conserved in TK. Beyond the kinase domain, N- and C-terminal flanking tails show remarkable structural similarity across orthologues, even though sequence conservation is limited to individual residues and short motifs: a YD-motif in the N-terminal tail; a [R/K]H[R/K]RYY sequence, a R-7x-R motif and position -2 of the latter in the C-terminal tail. Motifs in the C-terminal tail consistently covary with the divergent functional motifs of TK, being part of its pseudokinase signature. Contrary with these general features, the putatively inhibitory interaction of the catalytic aspartate with a tyrosine from loop P+1 is primarily confined to mammals. Finally, based on sequence clustering analysis, we identify TK subgroupings and propose a classification of titin genes from fish into a and b isoforms (ttna and ttnb) that can assist future studies. A curated genomic annotation is provided.
000646263 536__ $$0G:(DE-HGF)POF4-6G3$$a6G3 - PETRA III (DESY) (POF4-6G3)$$cPOF4-6G3$$fPOF IV$$x0
000646263 588__ $$aDataset connected to DataCite
000646263 693__ $$0EXP:(DE-H253)P-P14-20150101$$1EXP:(DE-H253)PETRAIII-20150101$$6EXP:(DE-H253)P-P14-20150101$$aPETRA III$$fPETRA Beamline P14$$x0
000646263 7001_ $$aGravenhorst, Peter$$b1
000646263 7001_ $$0P:(DE-H253)PIP1019913$$aMayans, Olga$$b2$$eCorresponding author
000646263 773__ $$0PERI:(DE-600)2007050-0$$a10.1042/BCJ20253442$$gVol. 483, no. 01, p. 55 - 70$$n01$$p55 - 70$$tThe biochemical journal / Reviews$$v483$$x1470-8728$$y2026
000646263 8564_ $$uhttps://portlandpress.com/biochemj/article/483/01/55/236989/Molecular-identifiers-of-the-evolutionarily
000646263 8564_ $$uhttps://bib-pubdb1.desy.de/record/646263/files/Molecular%20identifiers%20of%20the%20evolutionarily%20conserved%20titin%20pseudokinase.pdf$$yOpenAccess
000646263 8564_ $$uhttps://bib-pubdb1.desy.de/record/646263/files/Molecular%20identifiers%20of%20the%20evolutionarily%20conserved%20titin%20pseudokinase.pdf?subformat=pdfa$$xpdfa$$yOpenAccess
000646263 909CO $$ooai:bib-pubdb1.desy.de:646263$$popenaire$$popen_access$$pVDB$$pdriver$$pdnbdelivery
000646263 9101_ $$0I:(DE-588b)235011-7$$6P:(DE-H253)PIP1121049$$aEuropean Molecular Biology Laboratory$$b0$$kEMBL
000646263 9101_ $$0I:(DE-HGF)0$$6P:(DE-H253)PIP1121049$$aExternal Institute$$b0$$kExtern
000646263 9101_ $$0I:(DE-588b)235011-7$$6P:(DE-H253)PIP1019913$$aEuropean Molecular Biology Laboratory$$b2$$kEMBL
000646263 9101_ $$0I:(DE-HGF)0$$6P:(DE-H253)PIP1019913$$aExternal Institute$$b2$$kExtern
000646263 9131_ $$0G:(DE-HGF)POF4-6G3$$1G:(DE-HGF)POF4-6G0$$2G:(DE-HGF)POF4-600$$3G:(DE-HGF)POF4$$4G:(DE-HGF)POF$$aDE-HGF$$bForschungsbereich Materie$$lGroßgeräte: Materie$$vPETRA III (DESY)$$x0
000646263 9141_ $$y2026
000646263 915__ $$0LIC:(DE-HGF)CCBY4$$2HGFVOC$$aCreative Commons Attribution CC BY 4.0
000646263 915__ $$0StatID:(DE-HGF)0200$$2StatID$$aDBCoverage$$bSCOPUS$$d2023-10-22
000646263 915__ $$0StatID:(DE-HGF)0160$$2StatID$$aDBCoverage$$bEssential Science Indicators$$d2023-10-22
000646263 915__ $$0StatID:(DE-HGF)1050$$2StatID$$aDBCoverage$$bBIOSIS Previews$$d2023-10-22
000646263 915__ $$0StatID:(DE-HGF)1190$$2StatID$$aDBCoverage$$bBiological Abstracts$$d2023-10-22
000646263 915__ $$0StatID:(DE-HGF)0600$$2StatID$$aDBCoverage$$bEbsco Academic Search$$d2023-10-22
000646263 915__ $$0StatID:(DE-HGF)0100$$2StatID$$aJCR$$bBIOCHEM J : 2022$$d2023-10-22
000646263 915__ $$0StatID:(DE-HGF)0113$$2StatID$$aWoS$$bScience Citation Index Expanded$$d2023-10-22
000646263 915__ $$0StatID:(DE-HGF)1030$$2StatID$$aDBCoverage$$bCurrent Contents - Life Sciences$$d2023-10-22
000646263 915__ $$0StatID:(DE-HGF)0150$$2StatID$$aDBCoverage$$bWeb of Science Core Collection$$d2023-10-22
000646263 915__ $$0StatID:(DE-HGF)9900$$2StatID$$aIF < 5$$d2023-10-22
000646263 915__ $$0StatID:(DE-HGF)0510$$2StatID$$aOpenAccess
000646263 915__ $$0StatID:(DE-HGF)0030$$2StatID$$aPeer Review$$bASC$$d2023-10-22
000646263 915__ $$0StatID:(DE-HGF)0300$$2StatID$$aDBCoverage$$bMedline$$d2023-10-22
000646263 915__ $$0StatID:(DE-HGF)0320$$2StatID$$aDBCoverage$$bPubMed Central$$d2023-10-22
000646263 915__ $$0StatID:(DE-HGF)0199$$2StatID$$aDBCoverage$$bClarivate Analytics Master Journal List$$d2023-10-22
000646263 9201_ $$0I:(DE-H253)EMBL-User-20120814$$kEMBL-User$$lEMBL-User$$x0
000646263 980__ $$ajournal
000646263 980__ $$aVDB
000646263 980__ $$aUNRESTRICTED
000646263 980__ $$aI:(DE-H253)EMBL-User-20120814
000646263 9801_ $$aFullTexts