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@ARTICLE{Stafflinger:644609,
      author       = {Stafflinger, Helena and Neißner, Konstantin and Bartsch,
                      Saskia and Pichler, Alexander K and Bartosik, Karolina and
                      Dhamotharan, Karthikeyan and Abele, Rupert and
                      Duchardt-Ferner, Elke and Micura, Ronald and Schindelin,
                      Hermann and Wöhnert, Jens},
      title        = {{C}rystal structure of the class {V} {GTP}-binding {RNA}
                      aptamer bound to its ligand: {GTP} recognition by a
                      topologically complex intermolecular {G}-quadruplex},
      journal      = {Nucleic acids symposium series},
      volume       = {53},
      number       = {22},
      issn         = {0305-1048},
      address      = {Oxford},
      publisher    = {Oxford Univ. Press},
      reportid     = {PUBDB-2026-00442},
      pages        = {gkaf1315},
      year         = {2025},
      note         = {DFG) grant WO 901/1-1 (to J.W.), DFG through Grant
                      INST161/816-1 FUGG},
      abstract     = {RNA aptamers obtained from in vitro selection experiments
                      provide unique insights into the structural properties and
                      ligand recognition capabilities of RNA in general. In order
                      to investigate the relationships between RNA sequence
                      information content and structural complexity with ligand
                      affinity and specificity, the Szostak group previously
                      identified eleven different families of GTP-binding aptamers
                      varying widely in their sequence conservation patterns as
                      well as in their secondary structure complexity and
                      topology. The class V GTP aptamer has a particularly high
                      affinity for GTP. Here, we report the high-resolution X-ray
                      crystal structure of this aptamer–GTP complex. Remarkably,
                      GTP is found to be integrated into one layer of a
                      two-layered G-quadruplex, thereby rationalizing the high
                      ligand affinity and the observed specificity. The
                      G-quadruplex is extended on one side by a noncanonical
                      tetrad layer containing three non-G nucleotides and on the
                      other side by a Watson–Crick base pair stacking on top of
                      an unpaired adenine. Interestingly, the G-nucleotides
                      forming the G-quadruplex originate from two asymmetric
                      bulges separated by an 8 base pair A-form helix, resulting
                      in a complex and previously unobserved G-quadruplex
                      topology.},
      cin          = {EMBL-User},
      ddc          = {540},
      cid          = {I:(DE-H253)EMBL-User-20120814},
      pnm          = {6G3 - PETRA III (DESY) (POF4-6G3)},
      pid          = {G:(DE-HGF)POF4-6G3},
      experiment   = {EXP:(DE-H253)P-P12-20150101 / EXP:(DE-H253)P-P13-20150101},
      typ          = {PUB:(DE-HGF)16},
      doi          = {10.1093/nar/gkaf1315},
      url          = {https://bib-pubdb1.desy.de/record/644609},
}