TY  - JOUR
AU  - Vaskan, I. S.
AU  - Petoukhov, M. V.
AU  - Bovin, N. V.
AU  - Ryzhov, I. M.
AU  - Dimitreva, V. A.
AU  - Shtykova, E. V.
AU  - Oleinikov, V. A.
AU  - Zalygin, A. V.
TI  - Structure of Supramers Formed by Glycolipid Analogues: SAXS Study
JO  - Bulletin of the Russian Academy of Sciences / Physics
VL  - 89
IS  - S2
SN  - 1062-8738
CY  - New York, NY
PB  - Allerton Press
M1  - PUBDB-2026-00439
SP  - S347 - S352
PY  - 2025
AB  - Synthetic glycolipids and similar amphiphils with peptide and other head groups have been designed for labeling/modification of living cells under mild physiological conditions. Under-standing the mechanism of their penetration through the cellular glycocalyx and subsequent insertion into the plasma membrane opens up the prospect of improving the recently found anti-tumor properties of such constructs. In this work, we applied small-angle X-ray scattering (SAXS) technique to characterize structure of nanoparticles formed by self-assembly of synthetic glycolipid A (type 2)-Ad-DE and to estimate its dependence on the glycolipid concentration. The studies were performed at a range of SAXS-applicable concentrations. The obtained results indicate that self-assembly process leads to formation of monodisperse nanoparticles with micelle-like architecture, which is maintained regardless of concentration, indicating absence of the nanoparticle’s positive interaction with their glycopart. We applied ab initio modeling that showed a good agreement with experimental data, and found that the ellipsoid monodisperse nanoparticles have a size of about 14 nm. Quasi-atomic modeling visualised that glycan ligands are well accessed for biological recognition. This knowledge will facilitate further study of the formation of the supramolecular form(s) of A(type 2)-Ad-DE and other glycolipids within the glycocalyx and its further fate in new therapeutic strategies.
LB  - PUB:(DE-HGF)16
DO  - DOI:10.1134/S1062873825714618
UR  - https://bib-pubdb1.desy.de/record/644606
ER  -