% IMPORTANT: The following is UTF-8 encoded.  This means that in the presence
% of non-ASCII characters, it will not work with BibTeX 0.99 or older.
% Instead, you should use an up-to-date BibTeX implementation like “bibtex8” or
% “biber”.

@INPROCEEDINGS{Grebinyk:643496,
      author       = {Grebinyk, Anna},
      title        = {{Z}ebrafish {H}usbandry at {FLASH}lab@{PITZ} in
                      {O}peration},
      reportid     = {PUBDB-2026-00243},
      year         = {2025},
      abstract     = {Background and Aims The $R\&D$ platform FLASHlab@PITZ
                      (Photo Injector Test facility at DESY in Zeuthen) aims to
                      provide the radiation oncology community with an in vitro
                      and in vivo infrastructure for systematic investigations of
                      electron irradiation at an extremely wide dose rate range
                      from conventional up to 1014 Gy/s. Between 2023 and 2025, a
                      dedicated infrastructure for in vitro and in vivo
                      experiments was established on site. The operation of
                      zebrafish husbandry was initiated in 2024 and is being
                      reported.Methods A modular biolab in container design was
                      installed on campus to accommodate biological research. In
                      August 2024, the first phase of the FLASHlab@PITZ animal
                      laboratory began operation with zebrafish (Danio rerio). The
                      Max Delbrück Center for Molecular Medicine had prepared a
                      genetically diverse starting wild-type AB group for
                      breeding. A fish care was established under standardized
                      husbandry protocols, including stable temperature, and
                      continuously monitored water chemistry to ensure a healthy
                      environment and reliable breeding. Breeding was done at a
                      1:2 male-to-female ratio in spawning tanks. The embryo
                      generation methodology was prepared to provide consistent
                      access for future experiments.Results A healthy and
                      genetically diverse zebrafish population is being maintained
                      with reliable embryo yield required for high-throughput
                      radiation testing. Husbandry and environmental parameters,
                      as well the breeding performance met the experimental
                      requirements.Conclusion With its newly established
                      infrastructure, FLASHlab@PITZ is ready to perform
                      standardized irradiation studies using zebrafish embryos
                      (≤ 120 hours post-fertilization) as a high-throughput
                      model of normal tissue sparing effect. Initial experiments
                      with conventional X-rays will establish a standardized
                      reference framework for comparison with subsequent electron
                      beam exposure studies which should help to provide a
                      selection of optimal irradiation parameters},
      month         = {Dec},
      date          = {2025-12-10},
      organization  = {FRPT 2025, Prague (Czech Republic), 10
                       Dec 2025 - 12 Dec 2025},
      cin          = {$Z_PITZ$},
      cid          = {$I:(DE-H253)Z_PITZ-20210408$},
      pnm          = {621 - Accelerator Research and Development (POF4-621)},
      pid          = {G:(DE-HGF)POF4-621},
      experiment   = {EXP:(DE-H253)PITZ-20150101},
      typ          = {PUB:(DE-HGF)1},
      url          = {https://bib-pubdb1.desy.de/record/643496},
}