TY  - CONF
AU  - Grebinyk, Anna
TI  - Zebrafish Husbandry at FLASHlab@PITZ in Operation
M1  - PUBDB-2026-00243
PY  - 2025
AB  - Background and Aims The R</td><td width="150">
AB  - D platform FLASHlab@PITZ (Photo Injector Test facility at DESY in Zeuthen) aims to provide the radiation oncology community with an in vitro and in vivo infrastructure for systematic investigations of electron irradiation at an extremely wide dose rate range from conventional up to 1014 Gy/s. Between 2023 and 2025, a dedicated infrastructure for in vitro and in vivo experiments was established on site. The operation of zebrafish husbandry was initiated in 2024 and is being reported.Methods A modular biolab in container design was installed on campus to accommodate biological research. In August 2024, the first phase of the FLASHlab@PITZ animal laboratory began operation with zebrafish (Danio rerio). The Max Delbrück Center for Molecular Medicine had prepared a genetically diverse starting wild-type AB group for breeding. A fish care was established under standardized husbandry protocols, including stable temperature, and continuously monitored water chemistry to ensure a healthy environment and reliable breeding. Breeding was done at a 1:2 male-to-female ratio in spawning tanks. The embryo generation methodology was prepared to provide consistent access for future experiments.Results A healthy and genetically diverse zebrafish population is being maintained with reliable embryo yield required for high-throughput radiation testing. Husbandry and environmental parameters, as well the breeding performance met the experimental requirements.Conclusion With its newly established infrastructure, FLASHlab@PITZ is ready to perform standardized irradiation studies using zebrafish embryos (≤ 120 hours post-fertilization) as a high-throughput model of normal tissue sparing effect. Initial experiments with conventional X-rays will establish a standardized reference framework for comparison with subsequent electron beam exposure studies which should help to provide a selection of optimal irradiation parameters
T2  - FRPT 2025
CY  - 10 Dec 2025 - 12 Dec 2025, Prague (Czech Republic)
Y2  - 10 Dec 2025 - 12 Dec 2025
M2  - Prague, Czech Republic
LB  - PUB:(DE-HGF)1
UR  - https://bib-pubdb1.desy.de/record/643496
ER  -