000643496 001__ 643496
000643496 005__ 20260114103700.0
000643496 037__ $$aPUBDB-2026-00243
000643496 1001_ $$0P:(DE-H253)PIP1103334$$aGrebinyk, Anna$$b0$$eCorresponding author
000643496 1112_ $$aFRPT 2025$$cPrague$$d2025-12-10 - 2025-12-12$$wCzech Republic
000643496 245__ $$aZebrafish Husbandry at FLASHlab@PITZ in Operation
000643496 260__ $$c2025
000643496 3367_ $$0PUB:(DE-HGF)1$$2PUB:(DE-HGF)$$aAbstract$$babstract$$mabstract$$s1768383253_3311981
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000643496 520__ $$aBackground and Aims The R&D platform FLASHlab@PITZ (Photo Injector Test facility at DESY in Zeuthen) aims to provide the radiation oncology community with an in vitro and in vivo infrastructure for systematic investigations of electron irradiation at an extremely wide dose rate range from conventional up to 1014 Gy/s. Between 2023 and 2025, a dedicated infrastructure for in vitro and in vivo experiments was established on site. The operation of zebrafish husbandry was initiated in 2024 and is being reported.Methods A modular biolab in container design was installed on campus to accommodate biological research. In August 2024, the first phase of the FLASHlab@PITZ animal laboratory began operation with zebrafish (Danio rerio). The Max Delbrück Center for Molecular Medicine had prepared a genetically diverse starting wild-type AB group for breeding. A fish care was established under standardized husbandry protocols, including stable temperature, and continuously monitored water chemistry to ensure a healthy environment and reliable breeding. Breeding was done at a 1:2 male-to-female ratio in spawning tanks. The embryo generation methodology was prepared to provide consistent access for future experiments.Results A healthy and genetically diverse zebrafish population is being maintained with reliable embryo yield required for high-throughput radiation testing. Husbandry and environmental parameters, as well the breeding performance met the experimental requirements.Conclusion With its newly established infrastructure, FLASHlab@PITZ is ready to perform standardized irradiation studies using zebrafish embryos (≤ 120 hours post-fertilization) as a high-throughput model of normal tissue sparing effect. Initial experiments with conventional X-rays will establish a standardized reference framework for comparison with subsequent electron beam exposure studies which should help to provide a selection of optimal irradiation parameters
000643496 536__ $$0G:(DE-HGF)POF4-621$$a621 - Accelerator Research and Development (POF4-621)$$cPOF4-621$$fPOF IV$$x0
000643496 693__ $$0EXP:(DE-H253)PITZ-20150101$$5EXP:(DE-H253)PITZ-20150101$$ePhoto Injector Test Facility$$x0
000643496 8564_ $$uhttps://bib-pubdb1.desy.de/record/643496/files/FRPT_Grebinyk_abstract.pdf$$yRestricted
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000643496 9101_ $$0I:(DE-588b)2008985-5$$6P:(DE-H253)PIP1103334$$aDeutsches Elektronen-Synchrotron$$b0$$kDESY
000643496 9131_ $$0G:(DE-HGF)POF4-621$$1G:(DE-HGF)POF4-620$$2G:(DE-HGF)POF4-600$$3G:(DE-HGF)POF4$$4G:(DE-HGF)POF$$aDE-HGF$$bForschungsbereich Materie$$lMaterie und Technologie$$vAccelerator Research and Development$$x0
000643496 9201_ $$0I:(DE-H253)Z_PITZ-20210408$$kZ_PITZ$$lTechnologie$$x0
000643496 980__ $$aabstract
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