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@ARTICLE{Myers:642942,
author = {Myers, Tanner M and Burnim, Andrew A and Jermain, Madison D
and Sondermann, Holger and Lee, Vincent T and Jiang,
Xiaofang and Winkler, Wade C.},
title = {{F}unctional analyses of bacterial {N}ano{RN}ase {B}
proteins reveals defining features of this enzyme family},
journal = {Nucleic acids symposium series},
volume = {53},
number = {22},
issn = {0305-1048},
address = {Oxford},
publisher = {Oxford Univ. Press},
reportid = {PUBDB-2025-05732},
pages = {gkaf1384},
year = {2025},
note = {ISSN 1362-4962 not unique: **2 hits**.},
abstract = {A combination of exoribonucleases and endoribonucleases
degrades RNA polymers to recycle nucleoside monophosphates.
A byproduct of these reactions is the accumulation of short
RNAs, 2–5 nucleotides in length. Characteristic enzymes,
generally referred to as nanoRNases, specifically process
short RNAs. Genes encoding nanoRNases are essential in some
bacteria; therefore, it is assumed that the accumulation of
short RNAs is detrimental to cells. However, the substrate
preferences and enzymatic mechanisms of the known categories
of nanoRNase enzymes have not been equally investigated. The
NrnB category of nanoRNases has been particularly
understudied. In this study, we identified bacterial NrnB
homologs and discovered they can be grouped into three
classes of proteins, which can be identified by their
characteristic sequence features. Purified representatives
of these classes of proteins revealed that they all process
RNA substrates from the 3′-terminus. The presence of
sequence features at the C-terminus was shown to be
diagnostic for general exoribonuclease activity against long
RNA substrates, whereas the absence of these C-terminal
elements was correlated with proteins that preferentially
acted against shorter RNA substrates. Together, these data
define members of the overall NrnB family of nanoRNase
proteins and identify some of their key features.},
cin = {CSSB-DESY-HS},
ddc = {540},
cid = {I:(DE-H253)CSSB-DESY-HS-20210521},
pnm = {633 - Life Sciences – Building Blocks of Life: Structure
and Function (POF4-633)},
pid = {G:(DE-HGF)POF4-633},
experiment = {EXP:(DE-MLZ)NOSPEC-20140101},
typ = {PUB:(DE-HGF)16},
doi = {10.1093/nar/gkaf1384},
url = {https://bib-pubdb1.desy.de/record/642942},
}