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@ARTICLE{Spiliopoulou:642768,
      author       = {Spiliopoulou, Maria and Hatton, Caitlin and Kollewe, Martin
                      and Leimkohl, Jan-Philipp and Schikora, Hendrik and
                      Tellkamp, Friedjof and Mehrabi, Pedram and Schulz, Eike},
      title        = {{S}pitrobot-2 advances time-resolved cryo-trapping
                      crystallography to under 25 ms},
      journal      = {Communications chemistry},
      volume       = {8},
      number       = {1},
      issn         = {2399-3669},
      address      = {[London]},
      publisher    = {Macmillan Publishers Limited, part of Springer Nature},
      reportid     = {PUBDB-2025-05617},
      pages        = {363},
      year         = {2025},
      abstract     = {We previously introduced the spitrobot, a protein crystal
                      plunging system that enables reaction quenching via
                      cryo-trapping with a time resolution in the millisecond
                      range. Here we present the next generation, spitrobot-2, as
                      an integrated benchtop device. User-friendliness has been
                      improved by semi-automatic sample exchange. Moreover, a
                      fully automated shutter shields the liquid nitrogen from the
                      humidified environment, improving sample integrity. Most
                      importantly, the cryo-trapping delay time has been reduced
                      to 23 ms, making spitrobot-2 twice as fast as the previous
                      generation. This further expands the number of target
                      systems that can be addressed by cryo-trapping time-resolved
                      crystallography. Using 12 crystal structures of three
                      independent model systems, we demonstrate successful
                      cryo-trapping via observation of conformational changes and
                      ligand binding within 25 ms. These improvements increase
                      the convenient access to cryo-trapping, time-resolved X-ray
                      crystallography empowering the MX community with efficient
                      tools to advance research in structural biology.},
      cin          = {EMBL-User},
      ddc          = {540},
      cid          = {I:(DE-H253)EMBL-User-20120814},
      pnm          = {6G3 - PETRA III (DESY) (POF4-6G3) / DFG project
                      G:(GEPRIS)451079909 - Untersuchung allosterischer
                      Mechanismen durch zeitaufgelöste serielle
                      Synchrotronkristallographie (451079909) / DFG project
                      G:(GEPRIS)458246365 - Zeitaufgelöste Strukturanalysde der
                      extended spectrum Beta-Lactamase CTX-M-14 (458246365) /
                      DynaPLIX - Dynamics of Protein–Ligand Interactions
                      (101071843)},
      pid          = {G:(DE-HGF)POF4-6G3 / G:(GEPRIS)451079909 /
                      G:(GEPRIS)458246365 / G:(EU-Grant)101071843},
      experiment   = {EXP:(DE-H253)P-P14-20150101},
      typ          = {PUB:(DE-HGF)16},
      doi          = {10.1038/s42004-025-01784-9},
      url          = {https://bib-pubdb1.desy.de/record/642768},
}