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@ARTICLE{Shafiei:642767,
      author       = {Shafiei, Alaleh and Baldir, Nilufer and Na, Jongbum and
                      Kim, Jin Kyun and DeMirci, Hasan},
      title        = {{C}omparative {S}tructural {A}nalysis of {E}scherichia
                      {C}oli {C}yay at {R}oom and {C}ryogenic {T}emperatures
                      {U}sing {M}acromolecular and {S}erial {C}rystallography},
      journal      = {ChemBioChem},
      volume       = {26},
      number       = {20},
      issn         = {1439-4227},
      address      = {Weinheim},
      publisher    = {Wiley-VCH},
      reportid     = {PUBDB-2025-05616},
      pages        = {e202500442},
      year         = {2025},
      abstract     = {Frataxin is a 23 kDa mitochondrial iron-binding protein
                      involved in the biogenesis of iron–sulfur (Fe–S)
                      clusters. Deficiency in frataxin is associated with
                      Friedreich's ataxia, a progressive neurodegenerative
                      disorder. CyaY, the bacterial ortholog of eukaryotic
                      frataxin, is believed to function as an iron donor in Fe–S
                      cluster assembly, making it a key target for structural and
                      functional studies. In this work, a comprehensive structural
                      analysis of the Escherichia coli CyaY protein is presented,
                      comparing its structure at room temperature and cryogenic
                      conditions. Notably, the first room-temperature structures
                      are obtained using the Turkish Light Source “Turkish
                      DeLight” X-ray diffractometer and serial synchrotron X-ray
                      crystallography, marking a significant step forward in
                      understanding CyaY under near-physiological conditions.},
      cin          = {EMBL-User},
      ddc          = {540},
      cid          = {I:(DE-H253)EMBL-User-20120814},
      pnm          = {6G3 - PETRA III (DESY) (POF4-6G3)},
      pid          = {G:(DE-HGF)POF4-6G3},
      experiment   = {EXP:(DE-H253)P-P14-20150101},
      typ          = {PUB:(DE-HGF)16},
      doi          = {10.1002/cbic.202500442},
      url          = {https://bib-pubdb1.desy.de/record/642767},
}