TY  - JOUR
AU  - Shafiei, Alaleh
AU  - Baldir, Nilufer
AU  - Na, Jongbum
AU  - Kim, Jin Kyun
AU  - DeMirci, Hasan
TI  - Comparative Structural Analysis of Escherichia Coli Cyay at Room and Cryogenic Temperatures Using Macromolecular and Serial Crystallography
JO  - ChemBioChem
VL  - 26
IS  - 20
SN  - 1439-4227
CY  - Weinheim
PB  - Wiley-VCH
M1  - PUBDB-2025-05616
SP  - e202500442
PY  - 2025
AB  - Frataxin is a 23 kDa mitochondrial iron-binding protein involved in the biogenesis of iron–sulfur (Fe–S) clusters. Deficiency in frataxin is associated with Friedreich's ataxia, a progressive neurodegenerative disorder. CyaY, the bacterial ortholog of eukaryotic frataxin, is believed to function as an iron donor in Fe–S cluster assembly, making it a key target for structural and functional studies. In this work, a comprehensive structural analysis of the Escherichia coli CyaY protein is presented, comparing its structure at room temperature and cryogenic conditions. Notably, the first room-temperature structures are obtained using the Turkish Light Source “Turkish DeLight” X-ray diffractometer and serial synchrotron X-ray crystallography, marking a significant step forward in understanding CyaY under near-physiological conditions.
LB  - PUB:(DE-HGF)16
DO  - DOI:10.1002/cbic.202500442
UR  - https://bib-pubdb1.desy.de/record/642767
ER  -