TY  - JOUR
AU  - Pazicky, Samuel
AU  - Tjia, Seth
AU  - Farias, Guilherme
AU  - Piwon, Nick
AU  - Philip, Nisha
AU  - Sobota, Radoslaw M.
AU  - Waters, Andrew P.
AU  - Gilberger, Tim-Wolf
AU  - Bozdech, Zbynek
TI  - MAP-X reveals distinct protein complex dynamics across Plasmodium falciparum blood stages
JO  - Nature microbiology
VL  - 10
IS  - 12
SN  - 2058-5276
CY  - London
PB  - Nature Publishing Group
M1  - PUBDB-2025-05272
SP  - 3229 - 3244
PY  - 2025
N1  - Waiting for fulltext 
AB  - The malaria parasite Plasmodium falciparum undergoes a complex intraerythrocytic developmental cycle (IDC) that relies on a dynamic network of protein–protein interactions. These are usually mapped ex vivo, limiting our understanding of their dynamics and composition in natural environments. Here we introduce the meltome-assisted profiling of protein complexes (MAP-X) that maps the complexome through thermal proteome profiling in intact cells. We applied MAP-X across seven timepoints in the P. falciparum IDC. MAP-X predicted more than 20,000 interactions, resolving conserved protein complexes, reproducing previously identified interactions and finding previously unreported associations. We found that malaria protein complexes undergo distinct dynamic alterations, and we predicted their moonlighting subunits that dissociate from their native complex to assume different biological functions. Altogether, our findings provide a resource for uncovering Plasmodium biology and show that MAP-X can characterize protein complexes in intact cells to reveal cellular physiology at a proteome-wide level.
LB  - PUB:(DE-HGF)16
DO  - DOI:10.1038/s41564-025-02173-7
UR  - https://bib-pubdb1.desy.de/record/641985
ER  -