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@ARTICLE{Sheernaly:641966,
author = {Sheernaly, Nandan and Steinbrueck, Axel and Krahn, Nicolas
and Rumancev, Christoph and Peeters, Frank and Jusufagic,
Lejla and Ochs, Jasmine and Garrevoet, Jan and Falkenberg,
Gerald and Rosenhahn, Axel and Metzler-Nolte, Nils},
title = {{I}nvestigating {M}etal and {F}luorophore {C}ontrolled
{I}ntracellular {L}ocalization in {N}oble {M}etal
{T}hiosemicarbazone {C}omplexes},
journal = {Chemistry - a European journal},
volume = {31},
number = {69},
issn = {0947-6539},
address = {Weinheim},
publisher = {Wiley-VCH},
reportid = {PUBDB-2025-05253},
pages = {e02613},
year = {2025},
note = {cc-byonline first},
abstract = {Transition metal complexes have been widely utilized as
cellular imaging tools. To impart organelle specificity,
ligand architecture is usually modified to modulate
properties like overall charge and lipophilicity. In many
such designs, the metal identity and its intrinsic
properties are often ignored. To address this gap, in this
study, we explored the effects of changing the metal center
on the localization patterns of isostructural complexes. To
this end, we employed the thiosemicarbazone Dp44mT to
synthesize coumarin-conjugated complexes of Au(III), Pt(II),
and Pd(II). Although the metal centers in these compounds
share a formal d8 configuration, they differ in properties
such as ionic radius, charge density, and ligand exchange
rates, which can affect their subcellular localization
patterns. In addition, we synthesized a second set of
analogous complexes using BODIPY as the conjugating
fluorophore to assess the influence of using a different dye
on the cellular distribution. Confocal imaging revealed that
the complexes exhibited distinct intracellular
distributions. For instance, while the coumarin-conjugated
Pt(II) complex localized specifically in lysosomes, the
corresponding lipophilic Pd(II) complex lacked this
specificity and instead followed a diffusely cytosolic
distribution. Similarly, the more lipophilic BODIPY
conjugated complexes were non-specific in their cellular
distribution as well. Overall, the findings of this study
highlight the interplay of metal identity and lipophilicity
in determining the localization patterns of Dp44mT-based
metal complexes, offering fresh insights into the design of
new metal-based imaging tools.},
cin = {DOOR ; HAS-User / FS-PETRA-S},
ddc = {660},
cid = {I:(DE-H253)HAS-User-20120731 /
I:(DE-H253)FS-PETRA-S-20210408},
pnm = {631 - Matter – Dynamics, Mechanisms and Control
(POF4-631) / 6G3 - PETRA III (DESY) (POF4-6G3) / DFG project
G:(GEPRIS)518777768 - Entwicklung von Sonden für das
selektive Anfärben intrazellulärer Organelle in der
Röntgenfluoreszenzspektroskopie von menschlichen Zell- und
Gewebeproben (518777768) / DFG project G:(GEPRIS)525502632 -
Ortsaufgelöste röntgenfluoreszenzanalytische Untersuchung
der intrazellulären Verteilung von Platin und Gold
basierten Zytostatika und Vergleich mit rutheniumbasierten
Wirkstoffen. (525502632) / GRK 2341 - GRK 2341: Mikrobielle
Substratumsetzung (321933041) / FS-Proposal: II-20230017
(II-20230017)},
pid = {G:(DE-HGF)POF4-631 / G:(DE-HGF)POF4-6G3 /
G:(GEPRIS)518777768 / G:(GEPRIS)525502632 /
G:(GEPRIS)321933041 / G:(DE-H253)II-20230017},
experiment = {EXP:(DE-H253)P-P06-20150101},
typ = {PUB:(DE-HGF)16},
doi = {10.1002/chem.202502613},
url = {https://bib-pubdb1.desy.de/record/641966},
}