000641384 001__ 641384
000641384 005__ 20251218212314.0
000641384 0247_ $$2doi$$a10.1038/s41598-025-25449-8
000641384 0247_ $$2datacite_doi$$a10.3204/PUBDB-2025-05045
000641384 037__ $$aPUBDB-2025-05045
000641384 041__ $$aEnglish
000641384 082__ $$a600
000641384 1001_ $$0P:(DE-H253)PIP1084548$$aZabelskii, Dmitrii$$b0$$eCorresponding author
000641384 245__ $$aViscoelastic characterization of the lipid cubic phase provides insights into high-viscosity extrusion injection for XFEL experiments
000641384 260__ $$a[London]$$bSpringer Nature$$c2025
000641384 3367_ $$2DRIVER$$aarticle
000641384 3367_ $$2DataCite$$aOutput Types/Journal article
000641384 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article$$bjournal$$mjournal$$s1766042753_1297603
000641384 3367_ $$2BibTeX$$aARTICLE
000641384 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000641384 3367_ $$00$$2EndNote$$aJournal Article
000641384 520__ $$aSerial crystallography (SX) is a powerful method for determining macromolecular structures, enabled by the advent of X-ray free-electron lasers (XFELs). SX experiments require the continuous delivery of microcrystals, achievable through liquid jets, high-viscosity extrusion (HVE) jets, and fixed-target methods. The lipid cubic phase (LCP) is a lipid-based medium commonly used for membrane protein crystallization and as a carrier medium for HVE injection. In this study, we present a rheometric characterization of LCP media and demonstrate that sample viscosity correlates well with the injection stability and, therefore, can effectively predict sample stability during HVE jetting. Using this approach, we determined the viscosity range corresponding to the stable, metastable, and unstable jetting regions. The critical viscosity values for the metastable and unstable jetting regions are 7 × 105 and 104 mPa⋅s, measured at the 0.3 s-1 shear rate. We show that ambient humidity is crucial for rheometric and fixed-target experiments involving LCP-embedded crystals. Specifically, LCP-embedded crystals rapidly lose diffraction quality when exposed to ambient humidity below 80%. Additionally, we demonstrate that sample viscosity measured in the rheometric experiment can help determine the necessary amount of stabilizing additive for HVE jet optimization. This approach was successfully tested on LCP mixed with long-chain polyethylene glycol and stabilized with Pluronic F-127 polymer.
000641384 536__ $$0G:(DE-HGF)POF4-6G13$$a6G13 - Accelerator of European XFEL (POF4-6G13)$$cPOF4-6G13$$fPOF IV$$x0
000641384 588__ $$aDataset connected to CrossRef, Journals: bib-pubdb1.desy.de
000641384 693__ $$0EXP:(DE-H253)XFEL-Exp-20150101$$1EXP:(DE-H253)XFEL-20150101$$5EXP:(DE-H253)XFEL-Exp-20150101$$aXFEL$$eExperiments at XFEL$$x0
000641384 693__ $$0EXP:(DE-H253)P-P14-20150101$$1EXP:(DE-H253)PETRAIII-20150101$$6EXP:(DE-H253)P-P14-20150101$$aPETRA III$$fPETRA Beamline P14$$x1
000641384 7001_ $$aRound, Ekaterina$$b1
000641384 7001_ $$aHan, Huijong$$b2
000641384 7001_ $$avon Stetten, David$$b3
000641384 7001_ $$aLetrun, Romain$$b4
000641384 7001_ $$aKim, Chan$$b5
000641384 7001_ $$aSato, Tokushi$$b6
000641384 7001_ $$aV. M. Melo, Diogo$$b7
000641384 7001_ $$ade Wijn, Raphaël$$b8
000641384 7001_ $$aKharitonov, Konstantin$$b9
000641384 7001_ $$aSmyth, Peter$$b10
000641384 7001_ $$aDoerner, Katerina$$b11
000641384 7001_ $$aKloos, Marco$$b12
000641384 7001_ $$aDietze, Thomas$$b13
000641384 7001_ $$aMorillo, Luis Lopez$$b14
000641384 7001_ $$aBean, Richard$$b15
000641384 7001_ $$aRound, Adam$$b16
000641384 773__ $$0PERI:(DE-600)2615211-3$$a10.1038/s41598-025-25449-8$$gVol. 15, no. 1, p. 38999$$n1$$p38999$$tScientific reports$$v15$$x2045-2322$$y2025
000641384 8564_ $$uhttps://bib-pubdb1.desy.de/record/641384/files/20251119151738628.pdf
000641384 8564_ $$uhttps://bib-pubdb1.desy.de/record/641384/files/20251119151738628.pdf?subformat=pdfa$$xpdfa
000641384 8564_ $$uhttps://bib-pubdb1.desy.de/record/641384/files/s41598-025-25449-8.pdf$$yOpenAccess
000641384 8564_ $$uhttps://bib-pubdb1.desy.de/record/641384/files/s41598-025-25449-8.pdf?subformat=pdfa$$xpdfa$$yOpenAccess
000641384 8767_ $$92025-11-04$$d2025-11-19$$eAPC$$jDEAL$$lSpringerNature$$zXFEL, keine Angabe DFG Projekt im Artikel
000641384 8767_ $$d2025-11-19$$ePayment fee$$jBestellt$$lSpringerNature$$zDEAS Gebühr
000641384 909CO $$ooai:bib-pubdb1.desy.de:641384$$popenaire$$popen_access$$pOpenAPC$$pdriver$$pVDB$$popenCost$$pdnbdelivery
000641384 9101_ $$0I:(DE-588)1043621512$$6P:(DE-H253)PIP1084548$$aEuropean XFEL$$b0$$kXFEL.EU
000641384 9131_ $$0G:(DE-HGF)POF4-6G13$$1G:(DE-HGF)POF4-6G0$$2G:(DE-HGF)POF4-600$$3G:(DE-HGF)POF4$$4G:(DE-HGF)POF$$aDE-HGF$$bForschungsbereich Materie$$lGroßgeräte: Materie$$vAccelerator of European XFEL$$x0
000641384 9141_ $$y2025
000641384 915pc $$0PC:(DE-HGF)0000$$2APC$$aAPC keys set
000641384 915pc $$0PC:(DE-HGF)0001$$2APC$$aLocal Funding
000641384 915pc $$0PC:(DE-HGF)0178$$2APC$$aDEAL: Springer Nature 01.01.2024
000641384 915pc $$0PC:(DE-HGF)0003$$2APC$$aDOAJ Journal
000641384 915__ $$0StatID:(DE-HGF)0200$$2StatID$$aDBCoverage$$bSCOPUS$$d2024-12-18
000641384 915__ $$0StatID:(DE-HGF)0160$$2StatID$$aDBCoverage$$bEssential Science Indicators$$d2024-12-18
000641384 915__ $$0StatID:(DE-HGF)1050$$2StatID$$aDBCoverage$$bBIOSIS Previews$$d2024-12-18
000641384 915__ $$0StatID:(DE-HGF)1190$$2StatID$$aDBCoverage$$bBiological Abstracts$$d2024-12-18
000641384 915__ $$0StatID:(DE-HGF)0600$$2StatID$$aDBCoverage$$bEbsco Academic Search$$d2024-12-18
000641384 915__ $$0StatID:(DE-HGF)1040$$2StatID$$aDBCoverage$$bZoological Record$$d2024-12-18
000641384 915__ $$0StatID:(DE-HGF)0100$$2StatID$$aJCR$$bSCI REP-UK : 2022$$d2024-12-18
000641384 915__ $$0StatID:(DE-HGF)0501$$2StatID$$aDBCoverage$$bDOAJ Seal$$d2024-07-29T15:28:26Z
000641384 915__ $$0StatID:(DE-HGF)0500$$2StatID$$aDBCoverage$$bDOAJ$$d2024-07-29T15:28:26Z
000641384 915__ $$0StatID:(DE-HGF)0113$$2StatID$$aWoS$$bScience Citation Index Expanded$$d2024-12-18
000641384 915__ $$0StatID:(DE-HGF)0700$$2StatID$$aFees$$d2024-12-18
000641384 915__ $$0StatID:(DE-HGF)0150$$2StatID$$aDBCoverage$$bWeb of Science Core Collection$$d2024-12-18
000641384 915__ $$0StatID:(DE-HGF)9900$$2StatID$$aIF < 5$$d2024-12-18
000641384 915__ $$0StatID:(DE-HGF)0510$$2StatID$$aOpenAccess
000641384 915__ $$0StatID:(DE-HGF)0030$$2StatID$$aPeer Review$$bASC$$d2024-12-18
000641384 915__ $$0StatID:(DE-HGF)0561$$2StatID$$aArticle Processing Charges$$d2024-12-18
000641384 915__ $$0StatID:(DE-HGF)1150$$2StatID$$aDBCoverage$$bCurrent Contents - Physical, Chemical and Earth Sciences$$d2024-12-18
000641384 915__ $$0StatID:(DE-HGF)0300$$2StatID$$aDBCoverage$$bMedline$$d2024-12-18
000641384 915__ $$0LIC:(DE-HGF)CCBY4$$2HGFVOC$$aCreative Commons Attribution CC BY 4.0
000641384 915__ $$0StatID:(DE-HGF)0199$$2StatID$$aDBCoverage$$bClarivate Analytics Master Journal List$$d2024-12-18
000641384 9201_ $$0I:(DE-H253)XFEL_E1_SPB_SFX-20210408$$kXFEL_E1_SPB/SFX$$lSPB/SFX$$x0
000641384 9201_ $$0I:(DE-H253)EMBL-User-20120814$$kEMBL-User$$lEMBL-User$$x1
000641384 980__ $$ajournal
000641384 980__ $$aVDB
000641384 980__ $$aI:(DE-H253)XFEL_E1_SPB_SFX-20210408
000641384 980__ $$aI:(DE-H253)EMBL-User-20120814
000641384 980__ $$aAPC
000641384 980__ $$aUNRESTRICTED
000641384 9801_ $$aAPC
000641384 9801_ $$aFullTexts