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@ARTICLE{Schmidt:639478,
      author       = {Schmidt, Christina and Lorenzen, Kristina and Schulz,
                      Joachim and Han, Huijong},
      title        = {{S}tandard {S}ample {P}reparation for {S}erial
                      {F}emtosecond {C}rystallography},
      journal      = {Biomolecules},
      volume       = {15},
      number       = {11},
      issn         = {2218-273X},
      address      = {Basel},
      publisher    = {MDPI},
      reportid     = {PUBDB-2025-04564},
      pages        = {1488},
      year         = {2025},
      abstract     = {AbstractThe development of serial crystallography (SX),
                      including serial synchrotron crystallography (SSX) at
                      synchrotron sources and serial femtosecond crystallography
                      (SFX) at X-ray free-electron lasers (XFELs), has facilitated
                      the collection of high-resolution diffraction data from
                      micron-sized crystals, providing unique insights into the
                      structures and dynamics of biomolecules at room temperature.
                      Standard samples are essential for the commissioning of new
                      XFEL instruments and the validation of experimental setups.
                      In this review, we summarize currently used standard
                      proteins and describe representative microcrystal
                      preparation workflows for four widely adopted models,
                      lysozyme, myoglobin, iq-mEmerald, and photoactive yellow
                      protein (PYP), drawing on established methodologies and
                      accumulated experience from their applications at the
                      European XFEL. By consolidating existing knowledge and
                      integrating protocols that have been systematically refined
                      and optimized through our experimental efforts, this review
                      aims to provide practical guidance for the serial
                      crystallography community, thereby enhancing reproducibility
                      and ensuring consistent experimental performance across
                      facilities.Keywords:serial femtosecond crystallography;
                      microcrystals; standard samples; protocol},
      cin          = {$XFEL_E2_SEC$},
      ddc          = {570},
      cid          = {$I:(DE-H253)XFEL_E2_SEC-20210408$},
      pnm          = {6G13 - Accelerator of European XFEL (POF4-6G13)},
      pid          = {G:(DE-HGF)POF4-6G13},
      experiment   = {EXP:(DE-H253)XFEL(machine)-20150101},
      typ          = {PUB:(DE-HGF)16},
      doi          = {10.3390/biom15111488},
      url          = {https://bib-pubdb1.desy.de/record/639478},
}