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@ARTICLE{Nopens:639446,
      author       = {Nopens, Martin and Greving, Imke and Flenner, Silja and
                      Hesse, Linnea and Lüdtke, Jan and Altgen, Michael and Koch,
                      Gerald and Beruda, Johannes and Heldner, Sabrina and Köhm,
                      Hannes and Kaschuro, Sergej and Olbrich, Andrea and Mietner,
                      Jakob Benedikt and Scheckenbach, Fabian and Sieburg-Rockel,
                      Jördis and Krause, Andreas},
      title        = {{D}esign and implementation of a climate chamber for
                      moisture sensitive nanotomography of biological samples},
      journal      = {Journal of synchrotron radiation},
      volume       = {32},
      number       = {5},
      issn         = {0909-0495},
      address      = {Chester},
      publisher    = {IUCr},
      reportid     = {PUBDB-2025-04534},
      pages        = {1354 - 1360},
      year         = {2025},
      abstract     = {Deep understanding of the structural composition and growth
                      of biological specimens is becoming increasingly important
                      for the development of bio-based and sustainable material
                      systems. Full-field nano-computed tomography is particularly
                      suitable for this purpose as it allows for non-destructive
                      3D imaging at high spatial resolution. However, most
                      biological samples are functionalized by water and respond
                      sensitively to any changes in climate conditions,
                      specifically relative humidity, by adjusting their material
                      moisture content. To date, only a limited number of
                      tomography instruments offer an in situ climate control
                      option to users. These, however, are limited either by the
                      range of relative humidity states, the long times required
                      to change the climate state, or obstruction or attenuation
                      of the beam. Here, the first fully automatized climate cell
                      for in situ full-field nanotomography is presented. It has
                      been designed, built and integrated at the nanotomography
                      station at the P05 imaging beamline, operated by Hereon at
                      the DESY storage ring PETRA III, Germany. The highly
                      flexible and windowless design allows the humidity dependent
                      swelling and shrinking of lignified plant cell walls to be
                      studied in situ, using phase contrast nanotomography. The
                      concept of this climate chamber can easily be integrated
                      into other setups. It operates in the relative humidity
                      range of $0–90\%$ and can be controlled in a temperature
                      range of 10–50°C. Climate conditions can be adjusted at
                      any time, remotely from the control hutch by using a
                      humidity generator. Results show that the developed setup
                      maintains a stable climate during the entire duration of a
                      tomographic scan at different humidities and does not
                      obstruct the sample or hinder the imaging conditions. During
                      the tomographic investigation the sample remains stable in
                      the flow of the air stream and shows typical cell wall
                      swelling and shrinking behaviour depending on the
                      equilibrium moisture content. This new climate cell is now
                      available to all users of the P05 nanotomography instrument
                      for conditioning samples, serving a wide range of scientific
                      applications.},
      cin          = {DOOR ; HAS-User / Hereon},
      ddc          = {550},
      cid          = {I:(DE-H253)HAS-User-20120731 / I:(DE-H253)Hereon-20210428},
      pnm          = {6G3 - PETRA III (DESY) (POF4-6G3) / SFB 986 Z02 -
                      Multiskalige Analyse von Strukturen und Prozessen mit
                      Synchrotronstrahlung und Neutronen (Z02) (221133217) /
                      FS-Proposal: II-20200789 (II-20200789)},
      pid          = {G:(DE-HGF)POF4-6G3 / G:(GEPRIS)221133217 /
                      G:(DE-H253)II-20200789},
      experiment   = {EXP:(DE-H253)P-P05-20150101},
      typ          = {PUB:(DE-HGF)16},
      doi          = {10.1107/S1600577525006484},
      url          = {https://bib-pubdb1.desy.de/record/639446},
}