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@ARTICLE{Dummunee:639307,
      author       = {Dummunee, Krittika and Parry, Rhys H. and Redecke, Lars and
                      Varjak, Margus and Brennan, Benjamin and Kohl, Alain and
                      McFarlane, Melanie},
      title        = {{T}he catalytic tetrad of {A}edes aegypti argonaute 2 is
                      critical for the antiviral activity of the exogenous si{RNA}
                      pathway},
      journal      = {JBC papers in press},
      volume       = {301},
      number       = {4},
      issn         = {0021-9258},
      address      = {Bethesda, MD},
      publisher    = {American Soc. for Biochemistry and Molecular Biology},
      reportid     = {PUBDB-2025-04408},
      pages        = {108332},
      year         = {2025},
      note         = {ISSN 0021-9258 not unique: **2 hits**.},
      abstract     = {Viruses transmitted by biting arthropods, arboviruses, pose
                      a significant global health and economic threat. Climate
                      change is exacerbating this issue by expanding the range of
                      disease-carrying vectors. Effective control of arbovirus
                      transmission often relies on targeting the vectors, making
                      it crucial to understand the interactions between the virus
                      and its vector. The exogenous siRNA (exo-siRNA) pathway is a
                      key antiviral defense mechanism in mosquitoes such as Aedes
                      aegypti. Argonaute 2 (Ago2) is a central protein in this
                      pathway, responsible for antiviral activity. While the PIWI
                      domain of Ago proteins is known to mediate slicing activity,
                      not all Ago proteins possess this slicing function. To
                      understand the antiviral mechanism of Ago2 in Ae. aegypti,
                      we aimed to confirm the presence of the catalytic tetrad, a
                      group of amino acids known to be crucial for slicing
                      activity. Here, we confirmed the tetrad (D740, E780, D812,
                      and H950) in Ae. aegypti Ago2 and demonstrated its essential
                      role in antiviral and siRNA pathway activity. Our findings
                      show that the catalytic tetrad is necessary for the
                      degradation of siRNA passenger strands. When the tetrad is
                      absent, siRNA duplexes accumulate, leading to a loss of
                      siRNA pathway function. This underscores the critical role
                      of the tetrad in the antiviral defense mechanism of Ae.
                      aegypti.},
      cin          = {FS DOOR-User / FS-PS},
      ddc          = {610},
      cid          = {$I:(DE-H253)FS_DOOR-User-20241023$ /
                      I:(DE-H253)FS-PS-20131107},
      pnm          = {633 - Life Sciences – Building Blocks of Life: Structure
                      and Function (POF4-633)},
      pid          = {G:(DE-HGF)POF4-633},
      experiment   = {EXP:(DE-MLZ)NOSPEC-20140101},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:39984048},
      doi          = {10.1016/j.jbc.2025.108332},
      url          = {https://bib-pubdb1.desy.de/record/639307},
}