% IMPORTANT: The following is UTF-8 encoded.  This means that in the presence
% of non-ASCII characters, it will not work with BibTeX 0.99 or older.
% Instead, you should use an up-to-date BibTeX implementation like “bibtex8” or
% “biber”.

@ARTICLE{Chepurna:639305,
      author       = {Chepurna, Oksana and Yakovliev, Artem and Ziniuk, Roman and
                      Grebinyk, Anna and Xu, Hao and Nikolaeva, Olena A. and
                      Marynin, Andrii I. and Vretik, Liudmyla O. and Qu, Junle and
                      Ohulchanskyy, Tymish Y.},
      title        = {{P}olymeric nanoparticles with a thermoresponsive shell
                      loaded with fluorescent molecules allow for thermally
                      enhanced fluorescence imaging and singlet oxygen generation},
      journal      = {Nanoscale advances},
      volume       = {7},
      number       = {7},
      issn         = {2516-0230},
      address      = {Cambridge},
      publisher    = {Royal Society of Chemistry},
      reportid     = {PUBDB-2025-04406},
      pages        = {1946 - 1961},
      year         = {2025},
      abstract     = {A thermosensitive polymeric nanoformulation (NF) was
                      fabricated for thermally enhanced near-infrared (NIR)
                      fluorescence imaging (FLI). It comprised core–shell
                      nanoparticles (NPs) with a polystyrene core and a
                      thermosensitive shell of a co-polymer of
                      N-isopropylacrylamide and acrylamide [poly(NIPAM-co-AA)],
                      which underwent a reversible conformational transition at
                      38–40 °C (corresponding to a lower critical solution
                      temperature, LCST), leading to a reversible shrinkage of NPs
                      from ∼250 nm to ∼140 nm for temperatures above LCST. The
                      NIR dye 3782SL or photosensitizer HPPH were loaded to the NP
                      shells. While the fluorescence of 3782SL and HPPH was
                      quenched in water, it recovered in the NPs dispersion as a
                      result of adsorption by NPs. Fluorescence for 3782SL and
                      HPPH in NF increased when the temperature increased above
                      LCST. Heating of HPPH-loaded NFs led to the elongation of
                      the HPPH fluorescence lifetime and increased the generation
                      of singlet oxygen ($^1$O$_2$). This occurred as a result of
                      the NP shrinkage, corresponding shell compaction and NP
                      aggregation, which hindered the internal conversion for
                      photoexcited molecules adsorbed by NPs, and resulted in an
                      increase in other deactivation pathways, namely fluorescence
                      emission and intersystem crossing. The latter led to an
                      increase in the triplet yield and, consequently, in singlet
                      oxygen generation. Fluorescence microscopy revealed a
                      2–3-fold increase in the 3782SL or HPPH fluorescence
                      signal from the NF-treated cells after they were heated up
                      to 40 °C. Comparable results were obtained for the FLI of
                      mice in vivo, after subcutaneous, intravenous, or
                      intratumoral NF injections and localized heating by NIR (1.3
                      μm) laser irradiation. The developed NF holds immense
                      potential for thermally enhanced FLI and photodynamic
                      therapy.},
      cin          = {$Z_PITZ$},
      ddc          = {540},
      cid          = {$I:(DE-H253)Z_PITZ-20210408$},
      pnm          = {621 - Accelerator Research and Development (POF4-621)},
      pid          = {G:(DE-HGF)POF4-621},
      experiment   = {EXP:(DE-MLZ)NOSPEC-20140101},
      typ          = {PUB:(DE-HGF)16},
      doi          = {10.1039/D4NA00687A},
      url          = {https://bib-pubdb1.desy.de/record/639305},
}