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@ARTICLE{Schnherr:639244,
      author       = {Schönherr, Robert and Eichler, Nina and Sornaly, Fatama A.
                      and Boger, Juliane and Frevert, Anne M. and Lahey-Rudolph,
                      Janine Mia and Meyer, Hannah and Weymar, Lisa and Redecke,
                      Lars},
      title        = {{I}ntracellular protein crystallization in living insect
                      cells},
      journal      = {FEBS Open Bio},
      volume       = {15},
      number       = {4},
      issn         = {2211-5463},
      address      = {Hoboken, NJ},
      publisher    = {Wiley},
      reportid     = {PUBDB-2025-04364},
      pages        = {551 - 562},
      year         = {2025},
      abstract     = {Crystallization of recombinant proteins in living cells is
                      an emerging approach complementing conventional
                      crystallization techniques. Homogeneous microcrystals well
                      suited for serial diffraction experiments at X-ray
                      free-electron lasers and synchrotron sources can be produced
                      in a quasi-native environment, without the need for target
                      protein purification. Several protein structures have
                      already been solved; however, exploiting the full potential
                      of this approach requires a systematic and versatile
                      screening strategy for intracellular crystal growth.
                      Recently, we published InCellCryst, a streamlined pipeline
                      for producing microcrystals within living insect cells.
                      Here, we present the detailed protocol, including optimized
                      target gene expression using a baculovirus vector system,
                      crystal formation, detection, and serial X-ray diffraction
                      directly in the cells. The specific environment within the
                      different cellular compartments acts as a screening
                      parameter to maximize the probability of crystal growth. If
                      successful, diffraction data can be collected 24 days
                      after the start of target gene cloning.},
      cin          = {FS-PS / U Lübeck},
      ddc          = {570},
      cid          = {I:(DE-H253)FS-PS-20131107 /
                      $I:(DE-H253)U_L__beck-20211012$},
      pnm          = {633 - Life Sciences – Building Blocks of Life: Structure
                      and Function (POF4-633) / DFG project G:(GEPRIS)49701054 -
                      EXC 306: Entzündungen an Grenzflächen (49701054)},
      pid          = {G:(DE-HGF)POF4-633 / G:(GEPRIS)49701054},
      experiment   = {EXP:(DE-MLZ)NOSPEC-20140101},
      typ          = {PUB:(DE-HGF)16},
      doi          = {10.1002/2211-5463.70020},
      url          = {https://bib-pubdb1.desy.de/record/639244},
}