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000638760 1001_ $$aEjby Hansen, Morten$$b0
000638760 245__ $$aUptake of fucosylated type I human milk oligosaccharide blocks by Bifidobacterium longum subsp. infantis
000638760 260__ $$aWashington, DC$$bAmerican Society for Microbiology$$c2025
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000638760 520__ $$aHuman milk oligosaccharides (HMOs) are uniquely rich in the type 1 building block disaccharide lacto-N-biose I (LNB; Galβ1,3GlcNAc), as compared to other mammals. Most HMOs are fucosylated, for example, α1,2 and α1,4 fucosylations on LNB blocks, resulting in H type 1 (H1) and Lewis a (Lea) epitopes, respectively. The dominance of Bifidobacterium in breastfed infant guts hinges on the efficient uptake of HMOs by specific ATP-binding cassette (ABC) importers. However, molecular insight into uptake of fucosylated LNB blocks is lacking. Here, we analyzed the uptake of LNB and its fucosylated H1 and Lea trisaccharides, as well as the mucin-derived disaccharide galacto-N-biose (GNB; Galβ1,3GalNAc) by an ABC importer from the HMO-utilization specialist Bifidobacterium longum subsp. infantis. Structural analyses and molecular dynamics simulations explained how fucosylated and non-fucosylated LNB forms are recognized with similar affinities by the binding protein of this importer. Strikingly, we showed that two ABC importers confer to the uptake of LNB, while the Lea trisaccharide is efficiently internalized by a single importer in B. infantis. Phylogenetic and structural analyses of bifidobacterial ABC-associated binding proteins showed that the Lea clade harbors homologs possessing internal cavities, which allows for the accommodation of branched oligosaccharides. Our work provides unique insight into the evolution and molecular basis of capture and uptake of key HMO and host-derived saccharide blocks, highlighting these compounds as hitherto unexplored candidates for fortification of infant formula.
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000638760 7001_ $$00000-0002-4223-0926$$aSakanaka, Mikiyasu$$b1
000638760 7001_ $$00000-0002-8760-6616$$aJensen, Mathias$$b2
000638760 7001_ $$aSakanaka, Hiroka$$b3
000638760 7001_ $$aPichler, Michael Jakob$$b4
000638760 7001_ $$aMaeda, Shingo$$b5
000638760 7001_ $$00000-0003-4762-5529$$aFranck Høvring, Julie$$b6
000638760 7001_ $$aNakajima, Aruto$$b7
000638760 7001_ $$aKunstmann, Sonja$$b8
000638760 7001_ $$aNielsen, Tine Sofie$$b9
000638760 7001_ $$0P:(DE-HGF)0$$aPeters, Günther Herbert Johannes$$b10
000638760 7001_ $$aSlotboom, Dirk Jan$$b11
000638760 7001_ $$aMorth, Jens Preben$$b12
000638760 7001_ $$00000-0003-4009-7874$$aKatayama, Takane$$b13
000638760 7001_ $$00000-0001-8250-1842$$aAbou Hachem, Maher$$b14$$eCorresponding author
000638760 773__ $$0PERI:(DE-600)2557172-2$$a10.1128/mbio.00368-25$$gVol. 16, no. 8, p. e00368-25$$n8$$pe00368-25$$tmBio$$v16$$x2161-2129$$y2025
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