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@ARTICLE{Skiba:637942,
author = {Skiba, Marvin and Klemeyer, Lars and Guedes, Gabriela and
Sun, Xiao and Haas, Sylvio and Cortajarena, Aitziber L. and
Koziej, Dorota and Parak, Wolfgang J. and Sanchez-Cano,
Carlos},
title = {{P}robing the {B}iological {I}dentity of {I}norganic
{N}anoparticles with {A}nomalous {S}mall {A}ngle {X}‐{R}ay
{S}cattering},
journal = {Small},
volume = {21},
number = {32},
issn = {1613-6810},
address = {Weinheim},
publisher = {Wiley-VCH},
reportid = {PUBDB-2025-03932},
pages = {2504135},
year = {2025},
abstract = {In a biological milieu, nanoparticles (NPs) undergo
alterations that go from aggregation or degradation to the
adsorption of a corona of proteins. Those changes influence
significantly the intrinsic properties and biological
capacities of the engineered nanomaterials and need to be
fully understood for the successful biomedical use of NPs.
Synchrotron anomalous small-angle X-ray scattering (ASAXS)
is used to probe the individual small-angle X-ray scattering
(SAXS) contributions from the different components of NPs
(core, polymeric coating, and protein corona) on several in
vitro systems. By applying a model-based fitting approach
combined with pair distance distribution analysis, it is
possible to investigate whether and to what extent proteins
formed a corona around different nanoparticles. The results
obtained agree with well-established dynamic light
scattering and transmission electron microscopy experiments,
confirming the capacity of ASAXS to obtain full information
from complex NP samples, and opening for its use in more
realistic biological environments.},
cin = {DOOR ; HAS-User / FS-PETRA-D},
ddc = {620},
cid = {I:(DE-H253)HAS-User-20120731 /
I:(DE-H253)FS-PETRA-D-20210408},
pnm = {633 - Life Sciences – Building Blocks of Life: Structure
and Function (POF4-633) / 6G3 - PETRA III (DESY) (POF4-6G3)},
pid = {G:(DE-HGF)POF4-633 / G:(DE-HGF)POF4-6G3},
experiment = {EXP:(DE-H253)P-P62-20221101},
typ = {PUB:(DE-HGF)16},
doi = {10.1002/smll.202504135},
url = {https://bib-pubdb1.desy.de/record/637942},
}