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000637345 1001_ $$aChan, Hsin-Ju$$b0
000637345 245__ $$aStructural Heterogeneity of Proteoform-Ligand Complexes in Adenosine Monophosphate-Activated Protein Kinase Uncovered by Integrated Top-Down Mass Spectrometry
000637345 260__ $$aWashington, DC$$bACS Publications$$c2025
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000637345 520__ $$aAdenosine monophosphate-activated protein kinase (AMPK) is a heterotrimeric complex (αβγ) that serves as a master regulator of cellular metabolism, making it a prominent drug target for various diseases. Post-translational modifications (PTMs) and ligand binding significantly affect the activity and function of AMPK. However, the dynamic interplay of PTMs, noncovalent interactions, and higher-order structures of the kinase complex remains poorly understood. Herein, we report for the first time the structural heterogeneity of the AMPK complex arising from ligand binding and proteoforms─protein products derived from PTMs, alternative splicing, and genetic variants─using integrated native and denatured top-down mass spectrometry (TDMS). The fully intact AMPK heterotrimeric complex exhibits heterogeneity due to phosphorylation and multiple adenosine monophosphate (AMP) binding states. Native TDMS delineates the subunit composition, AMP binding stoichiometry, and higher-order structure of AMPK complex, while denatured TDMS comprehensively characterizes the proteoforms and localizes the phosphorylation site. Notably, by integrating native TDMS and AlphaFold, we elucidate a flexibly connected regulatory region of AMPK β subunit that was previously unresolvable with traditional structural biology tools. Our findings offer new perspectives on protein kinase regulation and establish a versatile framework for comprehensive characterization of proteoform-ligand complexes. 
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000637345 536__ $$0G:(EU-Grant)101068151$$aTop-AMPK - Putting the top down on AMPK protein complex (101068151)$$c101068151$$fHORIZON-MSCA-2021-PF-01$$x1
000637345 536__ $$0G:(EU-Grant)759661$$aSPOCkS MS - Sampling Protein cOmplex Conformational Space with native top down Mass Spectrometry (759661)$$c759661$$fERC-2017-STG$$x2
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000637345 7001_ $$0P:(DE-H253)PIP1018823$$aKrichel, Boris$$b1
000637345 7001_ $$00009-0008-9587-7273$$aBandura, Liam J.$$b2
000637345 7001_ $$aChapman, Emily A.$$b3
000637345 7001_ $$00000-0003-0870-0127$$aRogers, Holden T.$$b4
000637345 7001_ $$00009-0005-7713-8205$$aFischer, Matthew S.$$b5
000637345 7001_ $$00000-0002-0478-4987$$aRoberts, David S.$$b6
000637345 7001_ $$aGao, Zhan$$b7
000637345 7001_ $$aWang, Man-Di$$b8
000637345 7001_ $$aWu, Jingshing$$b9
000637345 7001_ $$0P:(DE-H253)PIP1014042$$aUetrecht, Charlotte$$b10
000637345 7001_ $$00000-0001-8693-7010$$aJin, Song$$b11
000637345 7001_ $$0P:(DE-HGF)0$$aGe, Ying$$b12$$eCorresponding author
000637345 773__ $$0PERI:(DE-600)1472210-0$$a10.1021/jacs.5c06950$$gVol. 147, no. 34, p. 30809 - 30819$$n34$$p30809 - 30819$$tJournal of the American Chemical Society$$v147$$x0002-7863$$y2025
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