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@ARTICLE{Wittwer:636312,
author = {Wittwer, Felix and Anthuparambil, Nimmi Das and Unger,
Frederik and Gautam, Randeer Pratap and Flenner, Silja and
Greving, Imke and Gutt, Christian and Modregger, Peter},
title = {3{D} structure of protein networks and lipid globules in
heat-treated egg yolk revealed by x-ray holo-tomography},
journal = {Journal of food engineering},
volume = {410},
issn = {0260-8774},
address = {Amsterdam [u.a.]},
publisher = {Elsevier Science},
reportid = {PUBDB-2025-03651},
pages = {112916},
year = {2026},
note = {Additional funding: BMFTR (projects 05K24PSA, 05K22PS1,
05D23PS1, 05K25PS3, 05K25PS1) and DESY Center for Molecular
Water Science (CMWS)},
abstract = {Upon heating, egg yolk transforms from a liquid to a grainy
gel due to a combination of lipid aggregation, protein
denaturation-aggregation-gelation and other processes. This
heat-induced solidification of egg yolk can serve as a
useful model system for the investigation of protein
denaturation and gelation in biological systems in general.
Using x-ray holographic tomography, we studied the
heat-induced changes in egg yolk on the micron to sub-micron
length scale. In contrast to electron microscopy, x-ray
holography does not require sample staining, fixation, or
drying, which potentially alter the sample and harm sample
fidelity. Our results reveal a developing separation between
proteins and lipids with fatty components rapidly
aggregating into large globules around 40 µm in size.},
cin = {FS-PETRA / Hereon / FS-PET-S / DOOR ; HAS-User},
ddc = {640},
cid = {I:(DE-H253)FS-PETRA-20140814 / I:(DE-H253)Hereon-20210428 /
I:(DE-H253)FS-PET-S-20190712 / I:(DE-H253)HAS-User-20120731},
pnm = {633 - Life Sciences – Building Blocks of Life: Structure
and Function (POF4-633) / 6G3 - PETRA III (DESY) (POF4-6G3)
/ FS-Proposal: I-20240551 (I-20240551) / DFG project
G:(GEPRIS)460248799 - DAPHNE4NFDI - DAten aus PHoton- und
Neutronen Experimenten für NFDI (460248799)},
pid = {G:(DE-HGF)POF4-633 / G:(DE-HGF)POF4-6G3 /
G:(DE-H253)I-20240551 / G:(GEPRIS)460248799},
experiment = {EXP:(DE-H253)P-P05-20150101},
typ = {PUB:(DE-HGF)16},
doi = {10.1016/j.jfoodeng.2025.112916},
url = {https://bib-pubdb1.desy.de/record/636312},
}