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@ARTICLE{Dahlstroem:636039,
      author       = {Dahlstroem, Christian and Barezani, Johanna and Li, Jing
                      and Sopelniak, Kostiantyn and Muhs, Stefanie and Schneider,
                      Carola and Thünauer, Roland and Reimer, Rudolph and
                      Windhorst, Sabine},
      title        = {{M}echanism of {C}entrosomal {P}rotein 55 ({CEP}55)
                      {L}oading {I}nto {E}xosomes},
      journal      = {Journal of extracellular vesicles},
      volume       = {14},
      number       = {2},
      issn         = {2001-3078},
      address      = {Hoboken, NJ},
      publisher    = {Wiley},
      reportid     = {PUBDB-2025-03594},
      pages        = {e70046},
      year         = {2025},
      abstract     = {Up-regulation of Centrosomal Protein 55 (CEP55) in cancer
                      cells increases malignancy, and the protein can be
                      transferred via exosomes. However, the mechanism of how
                      CEP55 is delivered to exosomes is unknown. In this study, we
                      addressed this issue and analysed trafficking of EGFP-CEP55
                      from early to late endosomes by using high-resolution
                      microscopy. Our data show that endogenous as well as
                      EGFP-CEP55 appeared as dot-like structures in cancer cells.
                      However, we did not find an internalization of CEP55 into
                      early Rab5- and late Rab7-positive endosomes but only into
                      secretory late CD63-positive endosomes. In addition, an
                      association of the CEP55 dots with the endoplasmic reticulum
                      and with ALG-2-interacting protein X (Alix) dots was
                      detected. Moreover, mutation of the CEP55-Alix interaction
                      site strongly reduced the formation of CEP55 dots as well as
                      CEP55 localization in extracellular vesicles. In summary,
                      our data indicate that delivery of CEP55 into exosomes does
                      not occur by the canonical early-to-late endosome pathway
                      but by Alix-mediated recruitment to secretory late secretory
                      CD63 endosomes.},
      cin          = {CSSB-CF-ALFM},
      ddc          = {570},
      cid          = {I:(DE-H253)CSSB-CF-ALFM-20210629},
      pnm          = {899 - ohne Topic (POF4-899)},
      pid          = {G:(DE-HGF)POF4-899},
      experiment   = {EXP:(DE-H253)ALFM-20250101},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:39976236},
      doi          = {10.1002/jev2.70046},
      url          = {https://bib-pubdb1.desy.de/record/636039},
}