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@ARTICLE{Novack:635301,
      author       = {Novack, Gisela V. and Galeano, Pablo and Defelipe, Lucas A.
                      and Campanelli, Lorenzo and Campuzano, Karen S. and
                      Rotondaro, Cecilia and Castaño, Eduardo M. and Do Carmo,
                      Sonia and Cuello, A. Claudio and García-Alai, María M. and
                      Morelli, Laura},
      title        = {{T}he {S}upramolecular {A}rchitecture of {M}itochondrial
                      {C}omplex {I} in the {R}at {B}rain {I}s {A}ltered by
                      {A}lzheimers‐{L}ike {C}erebral {A}myloidosis},
      journal      = {Journal of neurochemistry},
      volume       = {169},
      number       = {2},
      issn         = {0022-3042},
      address      = {Oxford},
      publisher    = {Wiley-Blackwell},
      reportid     = {PUBDB-2025-03056},
      pages        = {e70017},
      year         = {2025},
      note         = {Should EMBL SPC papers also be entered into PUBDB, when no
                      beamline was used? And if yes, which POF should be chosen?
                      Thanks, Ivanka Waiting for fulltext},
      abstract     = {Mitochondrial respiratory complexes are organized into
                      supercomplexes (SC) to regulate electron flow and mitigate
                      oxidative stress. Alterations in SC organization in the
                      brain may affect energy expenditure, oxidative stress, and
                      neuronal survival. In this report, we investigated the
                      amount, activity and organization of mitochondrial complex I
                      (CI) in the hippocampus of 12-month-old McGill-R-Thy1-APP
                      transgenic (Tg) rats, an animal model of Alzheimer's-like
                      cerebral amyloidosis. By means of BN-PAGE, we found that the
                      organization of SC did not differ between genotypes, but a
                      lower abundance of SC was detected in Tg compared to
                      wild-type (WT) rats. Using a more sensitive technique (2-D
                      electrophoresis followed by Western blot), higher levels of
                      free CI and a decrease in the relative abundance of
                      assembled CI in SC (I-III2 and I-III2-IV) were observed in
                      Tg rats. In-gel activity assays showed that the total
                      activity of CI (CI + SC-CI) is lower in Tg compared to
                      WT, while Tg samples show a significant decrease in
                      SC-CI-associated activity. This alteration in CI assembly
                      was associated with nitro-oxidative stress and changes in
                      mitochondrial fusion-fission parameters. To assess CI
                      composition, we applied LC–MS/MS to the isolated CI from
                      BN-PAGE and found that 11 of 45 subunits described in
                      mammals were found to be less abundant in Tg. We examined
                      the levels of the nuclear-derived NDUFA9 subunit, which is
                      critical for CI assembly, and found higher levels in the
                      cytoplasmic fraction and lower levels in the mitochondrial
                      fraction in Tg, suggesting that brain amyloidosis affects
                      the import of CI subunits from the cytosol to the
                      mitochondria, destabilizing the SC. This is the first report
                      to characterize the types, abundance and activity of SC in
                      the hippocampus of an animal model of cerebral amyloidosis,
                      providing additional experimental evidence for the molecular
                      mechanisms underlying the brain bioenergetic deficit
                      characteristic of Alzheimer's disease.},
      cin          = {EMBL-User / EMBL},
      ddc          = {610},
      cid          = {I:(DE-H253)EMBL-User-20120814 / I:(DE-H253)EMBL-20120731},
      pnm          = {6G3 - PETRA III (DESY) (POF4-6G3)},
      pid          = {G:(DE-HGF)POF4-6G3},
      experiment   = {EXP:(DE-H253)SPC-20250101},
      typ          = {PUB:(DE-HGF)16},
      doi          = {10.1111/jnc.70017},
      url          = {https://bib-pubdb1.desy.de/record/635301},
}