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@ARTICLE{RajaSomu:634681,
author = {Raja Somu, Dawn and Soini, Steven A. and Briggs, Ani and
Singh, Kritika and Greving, Imke and Porter, Marianne and
Passerotti, Michelle and Merk, Vivian},
title = {{A} {N}anoscale {V}iew of the {S}tructure and {D}eformation
{M}echanism of {M}ineralized {S}hark {V}ertebral
{C}artilage},
journal = {ACS nano},
volume = {19},
number = {14},
issn = {1936-0851},
address = {Washington, DC},
publisher = {Soc.},
reportid = {PUBDB-2025-02595},
pages = {14410 - 14421},
year = {2025},
note = {Waiting for fulltext},
abstract = {Swimming kinematics and macroscale mechanical testing have
shown that the vertebral column of sharks acts as a
biological spring, storing and releasing energy during
locomotion. Using synchrotron X-ray nanotomography and
deep-learning image segmentation, we studied the
ultrastructure and deformation mechanism of mineralized
shark vertebrae from Carcharhinus limbatus (Blacktip shark).
The vertebral centrum con regions: the corpus calcareum, a
hypermineralized double cone, and the intermediale, blocks
of mineralized cartilage interspersed by unmineralized
arches. At the micron scale, mineralized cartilage has
previously been described as a 3D network of interconnected
mineral plates that vary in thickness and spacing. The
corpus calcareum consists of stacked, interconnected, curved
mineralized planes permeated by a network of organic
occlusions. The mineral network in the intermedialia
resembles trabecular bone, including thicker struts in the
direction opposite to the predominant biological strain. We
characterized collagenous fiber elements winding around
lacunar spaces in the intermedialia, and we hypothesize the
swirling arrangement and elasticity of the fibers to be
distributing stress. With little permanent deformation
detected in mineralized structures, it is likely that the
soft organic matrix is crucial for absorbing energy through
deformation, irreversible damage, and viscoelastic behavior.
In the corpus calcareum, cracks typically terminate toward
thick struts along the mineral planes, resembling the
microscale crack deflection and arrest mechanism found in
other staggered biocomposites, such as nacre or bone. Using
transmission electron microscopy (TEM), we observed
preferentially oriented, needlelike bioapatite crystallites
and d-band patterns of collagen type-II fibrils resulting
from intrafibrillar mineralization.},
cin = {DOOR ; HAS-User / Hereon},
ddc = {540},
cid = {I:(DE-H253)HAS-User-20120731 / I:(DE-H253)Hereon-20210428},
pnm = {6G3 - PETRA III (DESY) (POF4-6G3) / FS-Proposal: I-20211443
(I-20211443) / FS-Proposal: I-20220547 (I-20220547)},
pid = {G:(DE-HGF)POF4-6G3 / G:(DE-H253)I-20211443 /
G:(DE-H253)I-20220547},
experiment = {EXP:(DE-H253)P-P05-20150101},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:40191917},
doi = {10.1021/acsnano.5c02004},
url = {https://bib-pubdb1.desy.de/record/634681},
}