000633008 001__ 633008
000633008 005__ 20250723110123.0
000633008 0247_ $$2doi$$a10.1107/S1600577525003959
000633008 0247_ $$2ISSN$$a0909-0495
000633008 0247_ $$2ISSN$$a1600-5775
000633008 0247_ $$2datacite_doi$$a10.3204/PUBDB-2025-02334
000633008 0247_ $$2openalex$$aopenalex:W4411239783
000633008 037__ $$aPUBDB-2025-02334
000633008 041__ $$aEnglish
000633008 082__ $$a550
000633008 1001_ $$0P:(DE-H253)PIP1103945$$aDa Vela, Stefano$$b0
000633008 245__ $$aAF4-to-SAXS: expanded characterization of nanoparticles and proteins at the P12 BioSAXS beamline
000633008 260__ $$aChester$$bIUCr$$c2025
000633008 3367_ $$2DRIVER$$aarticle
000633008 3367_ $$2DataCite$$aOutput Types/Journal article
000633008 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article$$bjournal$$mjournal$$s1752225638_2516342
000633008 3367_ $$2BibTeX$$aARTICLE
000633008 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000633008 3367_ $$00$$2EndNote$$aJournal Article
000633008 520__ $$aBiological small-angle X-ray scattering (SAXS) is a versatile and powerful technique for investigating the structural and biophysical properties of biologically and pharmaceutically relevant macromolecules and nanoparticles. SAXS offers detailed insights into macromolecular composition, size, shape and internal structure, while addressing key aspects such as oligomeric state, stability, molecular interactions, and conformational flexibility. Recently, asymmetrical-flow field-flow fractionation (AF4) was successfully coupled to SAXS, enabling online size-based fractionation and analysis of polydisperse samples. This approach allows precise, size-dependent characterization, offering significant advancements in the study of polydisperse systems. We have integrated an AF4 device at the P12 beamline at the European Molecular Biology Laboratory and implemented technical adaptations allowing full automation to make the system suitable for routine user access. We provide streamlined workflows and troubleshooting resources for both novice and advanced SAXS users thereby equipping them with clear guidance on performing AF4–SAXS measurements. The general principles of our set-up are easily adaptable to other beamlines which have integrated (or are planning to integrate) a similar system.
000633008 536__ $$0G:(DE-HGF)POF4-6G3$$a6G3 - PETRA III (DESY) (POF4-6G3)$$cPOF4-6G3$$fPOF IV$$x0
000633008 588__ $$aDataset connected to CrossRef, Journals: bib-pubdb1.desy.de
000633008 693__ $$0EXP:(DE-H253)P-P12-20150101$$1EXP:(DE-H253)PETRAIII-20150101$$6EXP:(DE-H253)P-P12-20150101$$aPETRA III$$fPETRA Beamline P12$$x0
000633008 7001_ $$0P:(DE-HGF)0$$aBartels, Kim$$b1
000633008 7001_ $$aFranke, Daniel$$b2
000633008 7001_ $$00000-0001-7945-2218$$aSoloviov, Dymtro$$b3
000633008 7001_ $$0P:(DE-H253)PIP1080396$$aGraewert, Tobias Wilhelm$$b4
000633008 7001_ $$0P:(DE-H253)PIP1081930$$aMolodenskiy, Dmitry$$b5
000633008 7001_ $$aKolb, Bastian$$b6
000633008 7001_ $$aWilhelmy, Christoph$$b7
000633008 7001_ $$aDrexel, Roland$$b8
000633008 7001_ $$0P:(DE-H253)PIP1111638$$aMeier, Florian$$b9
000633008 7001_ $$0P:(DE-HGF)0$$aHaas, Heinrich$$b10
000633008 7001_ $$aLangguth, Peter$$b11
000633008 7001_ $$0P:(DE-HGF)0$$aGraewert, Melissa A.$$b12$$eCorresponding author
000633008 773__ $$0PERI:(DE-600)2021413-3$$a10.1107/S1600577525003959$$gVol. 32, no. 4, p. 971 - 985$$n4$$p971 - 985$$tJournal of synchrotron radiation$$v32$$x0909-0495$$y2025
000633008 8564_ $$uhttps://journals.iucr.org/s/issues/2025/04/00/ju5081/index.html
000633008 8564_ $$uhttps://bib-pubdb1.desy.de/record/633008/files/AF4%20to%20SAXS%20expanded%20characterization%20of%20nanoparticles%20and%20proteins%20at%20the%20P12%20BioSAXS%20beamline.pdf$$yOpenAccess
000633008 8564_ $$uhttps://bib-pubdb1.desy.de/record/633008/files/AF4%20to%20SAXS%20expanded%20characterization%20of%20nanoparticles%20and%20proteins%20at%20the%20P12%20BioSAXS%20beamline.pdf?subformat=pdfa$$xpdfa$$yOpenAccess
000633008 909CO $$ooai:bib-pubdb1.desy.de:633008$$pdnbdelivery$$pdriver$$pVDB$$popen_access$$popenaire
000633008 9101_ $$0I:(DE-588b)235011-7$$6P:(DE-H253)PIP1103945$$aEuropean Molecular Biology Laboratory$$b0$$kEMBL
000633008 9101_ $$0I:(DE-HGF)0$$6P:(DE-H253)PIP1080396$$aExternal Institute$$b4$$kExtern
000633008 9101_ $$0I:(DE-H253)_CSSB-20140311$$6P:(DE-H253)PIP1081930$$aCentre for Structural Systems Biology$$b5$$kCSSB
000633008 9101_ $$0I:(DE-HGF)0$$6P:(DE-H253)PIP1111638$$aExternal Institute$$b9$$kExtern
000633008 9101_ $$0I:(DE-588b)235011-7$$6P:(DE-HGF)0$$aEuropean Molecular Biology Laboratory$$b12$$kEMBL
000633008 9131_ $$0G:(DE-HGF)POF4-6G3$$1G:(DE-HGF)POF4-6G0$$2G:(DE-HGF)POF4-600$$3G:(DE-HGF)POF4$$4G:(DE-HGF)POF$$aDE-HGF$$bForschungsbereich Materie$$lGroßgeräte: Materie$$vPETRA III (DESY)$$x0
000633008 9141_ $$y2025
000633008 915__ $$0StatID:(DE-HGF)0200$$2StatID$$aDBCoverage$$bSCOPUS$$d2025-01-06
000633008 915__ $$0StatID:(DE-HGF)0160$$2StatID$$aDBCoverage$$bEssential Science Indicators$$d2025-01-06
000633008 915__ $$0LIC:(DE-HGF)CCBY4$$2HGFVOC$$aCreative Commons Attribution CC BY 4.0
000633008 915__ $$0StatID:(DE-HGF)0600$$2StatID$$aDBCoverage$$bEbsco Academic Search$$d2025-01-06
000633008 915__ $$0StatID:(DE-HGF)0100$$2StatID$$aJCR$$bJ SYNCHROTRON RADIAT : 2022$$d2025-01-06
000633008 915__ $$0StatID:(DE-HGF)3001$$2StatID$$aDEAL Wiley$$d2025-01-06$$wger
000633008 915__ $$0StatID:(DE-HGF)0500$$2StatID$$aDBCoverage$$bDOAJ$$d2023-03-08T13:56:53Z
000633008 915__ $$0StatID:(DE-HGF)0501$$2StatID$$aDBCoverage$$bDOAJ Seal$$d2023-03-08T13:56:53Z
000633008 915__ $$0StatID:(DE-HGF)0113$$2StatID$$aWoS$$bScience Citation Index Expanded$$d2025-01-06
000633008 915__ $$0StatID:(DE-HGF)0700$$2StatID$$aFees$$d2025-01-06
000633008 915__ $$0StatID:(DE-HGF)0150$$2StatID$$aDBCoverage$$bWeb of Science Core Collection$$d2025-01-06
000633008 915__ $$0StatID:(DE-HGF)9900$$2StatID$$aIF < 5$$d2025-01-06
000633008 915__ $$0StatID:(DE-HGF)0510$$2StatID$$aOpenAccess
000633008 915__ $$0StatID:(DE-HGF)0030$$2StatID$$aPeer Review$$bASC$$d2025-01-06
000633008 915__ $$0StatID:(DE-HGF)0561$$2StatID$$aArticle Processing Charges$$d2025-01-06
000633008 915__ $$0StatID:(DE-HGF)1150$$2StatID$$aDBCoverage$$bCurrent Contents - Physical, Chemical and Earth Sciences$$d2025-01-06
000633008 915__ $$0StatID:(DE-HGF)0300$$2StatID$$aDBCoverage$$bMedline$$d2025-01-06
000633008 915__ $$0StatID:(DE-HGF)0420$$2StatID$$aNationallizenz$$d2025-01-06$$wger
000633008 915__ $$0StatID:(DE-HGF)0199$$2StatID$$aDBCoverage$$bClarivate Analytics Master Journal List$$d2025-01-06
000633008 9201_ $$0I:(DE-H253)EMBL-User-20120814$$kEMBL-User$$lEMBL-User$$x0
000633008 9201_ $$0I:(DE-H253)EMBL-20120731$$kEMBL$$lEMBL$$x1
000633008 980__ $$ajournal
000633008 980__ $$aVDB
000633008 980__ $$aUNRESTRICTED
000633008 980__ $$aI:(DE-H253)EMBL-User-20120814
000633008 980__ $$aI:(DE-H253)EMBL-20120731
000633008 9801_ $$aFullTexts