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@ARTICLE{Kung:626598,
      author       = {Kung, Jocky Chun Kui and Kadek, Alan and Koelbel, Knut and
                      Bandelow, Steffi and Bari, Sadia and Buck, Jens and Caleman,
                      Carl and Commandeur, Jan and Damjanovic, Tomislav and
                      Dörner, Simon and Fahmy, Karim and Flacht, Lara and
                      Heidemann, Johannes and Huynh, Khon and Kopicki,
                      Janine-Denise and Krichel, Boris and Lockhauserbaeumer,
                      Julia and Lorenzen, Kristina and Lu, Yinfei and Pogan, Ronja
                      and Rehmann, Jasmin and Schamoni-Kast, Kira and Lucas,
                      Schwob and Schweikhard, Lutz and Springer, Sebastian and
                      Svensson, Pamela and Simke, Florian and Trinter, Florian and
                      Toleikis, Sven and Kierspel, Thomas and Uetrecht, Charlotte},
      title        = {{X}-ray {S}pectroscopy {M}eets {N}ative {M}ass
                      {S}pectrometry: {P}robing {G}as-phase {P}rotein {C}omplexes},
      journal      = {Physical chemistry, chemical physics},
      volume       = {27},
      issn         = {1463-9076},
      address      = {Cambridge},
      publisher    = {RSC Publ.},
      reportid     = {PUBDB-2025-01499},
      pages        = {13234 - 13242},
      year         = {2025},
      abstract     = {Gas-phase activation and dissociation studies of
                      biomolecules, proteins and their non-covalent complexes
                      using X-rays hold great promise for revealing new insights
                      into the structure and function of biological samples. This
                      is due to the unique properties of X-ray molecular
                      interactions, such as site-specific and rapid ionization. In
                      this perspective, we report and discuss the promise of first
                      proof-of-principle studies of X-ray-induced dissociation of
                      native biological samples ranging from small 17 kDa
                      monomeric proteins up to large 808 kDa non-covalent protein
                      assemblies conducted at a synchrotron (PETRA III) and a
                      free-electron laser (FLASH2). A commercially available
                      quadrupole time-of-flight mass spectrometer (Q-ToF2,
                      Micromass/Waters), modified for high-mass analysis by MS
                      Vision, was further adapted for integration with the open
                      ports at the corresponding beamlines. The protein complexes
                      were transferred natively into the gas phase via
                      nano-electrospray ionization and subsequently probed by
                      extreme ultraviolet (FLASH2) or soft X-ray (PETRA III)
                      radiation, in either their folded state or following
                      collision-induced activation in the gas phase. Depending on
                      the size of the biomolecule and the activation method,
                      protein fragmentation, dissociation, or enhanced ionization
                      were observed. Additionally, an extension of the setup by
                      ion mobility is described, which can serve as a powerful
                      tool for structural separation of biomolecules prior to
                      X-ray probing. The first experimental results are discussed
                      in the broader context of current and upcoming X-ray
                      sources, highlighting their potential for advancing
                      structural biology in the future.},
      cin          = {FS-PS / CSSB-LIV/DESY-CU / FS-BIG / FS-SXQM / FS-FLASH-O /
                      DOOR ; HAS-User},
      ddc          = {540},
      cid          = {I:(DE-H253)FS-PS-20131107 /
                      $I:(DE-H253)CSSB-LIV_DESY-CU-20220525$ /
                      I:(DE-H253)FS-BIG-20220318 / I:(DE-H253)FS-SXQM-20190201 /
                      I:(DE-H253)FS-FLASH-O-20160930 /
                      I:(DE-H253)HAS-User-20120731},
      pnm          = {633 - Life Sciences – Building Blocks of Life: Structure
                      and Function (POF4-633) / 6G3 - PETRA III (DESY) (POF4-6G3)
                      / 6G2 - FLASH (DESY) (POF4-6G2) / 05K22PSA - Verbundprojekt
                      05K2022 - 2021-05988 SAXFELS:
                      Kleinwinkel-Röntgen-Freie-Elektronenlaser-Streuung
                      (BMBF-05K22PSA) / DFG project G:(GEPRIS)509471550 - Dynamik
                      photoionisations-induzierter Prozesse in laser-präparierten
                      Molekülen in der Gasphase und der wässrigen Phase
                      (509471550) / SPOCkS MS - Sampling Protein cOmplex
                      Conformational Space with native top down Mass Spectrometry
                      (759661) / MS SPIDOC - Mass Spectrometry for Single Particle
                      Imaging of Dipole Oriented protein Complexes (801406) /
                      FS-Proposal: I-20221192 (I-20221192) / FS-Proposal:
                      F-20150009 (F-20150009) / FS-Proposal: F-20171103
                      (F-20171103) / FS-Proposal: I-20180927 (I-20180927) /
                      FS-Proposal: I-20190534 (I-20190534)},
      pid          = {G:(DE-HGF)POF4-633 / G:(DE-HGF)POF4-6G3 /
                      G:(DE-HGF)POF4-6G2 / G:(DE-Ds200)BMBF-05K22PSA /
                      G:(GEPRIS)509471550 / G:(EU-Grant)759661 /
                      G:(EU-Grant)801406 / G:(DE-H253)I-20221192 /
                      G:(DE-H253)F-20150009 / G:(DE-H253)F-20171103 /
                      G:(DE-H253)I-20180927 / G:(DE-H253)I-20190534},
      experiment   = {EXP:(DE-H253)P-P04-20150101 / EXP:(DE-H253)F-FL24-20150901},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:40304431},
      doi          = {10.1039/D5CP00604J},
      url          = {https://bib-pubdb1.desy.de/record/626598},
}