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| 001 | 622097 | ||
| 005 | 20250723173210.0 | ||
| 024 | 7 | _ | |a 10.1016/j.str.2023.12.006 |2 doi |
| 024 | 7 | _ | |a 0969-2126 |2 ISSN |
| 024 | 7 | _ | |a 1878-4186 |2 ISSN |
| 024 | 7 | _ | |a altmetric:158199661 |2 altmetric |
| 024 | 7 | _ | |a pmid:38181786 |2 pmid |
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| 037 | _ | _ | |a PUBDB-2025-00183 |
| 041 | _ | _ | |a English |
| 082 | _ | _ | |a 540 |
| 100 | 1 | _ | |a Sievers, Katharina |0 P:(DE-H253)PIP1099196 |b 0 |
| 245 | _ | _ | |a Structural and functional insights into tRNA recognition by human tRNA guanine transglycosylase |
| 260 | _ | _ | |a London [u.a.] |c 2024 |b Elsevier Science |
| 336 | 7 | _ | |a article |2 DRIVER |
| 336 | 7 | _ | |a Output Types/Journal article |2 DataCite |
| 336 | 7 | _ | |a Journal Article |b journal |m journal |0 PUB:(DE-HGF)16 |s 1745406303_3662842 |2 PUB:(DE-HGF) |
| 336 | 7 | _ | |a ARTICLE |2 BibTeX |
| 336 | 7 | _ | |a JOURNAL_ARTICLE |2 ORCID |
| 336 | 7 | _ | |a Journal Article |0 0 |2 EndNote |
| 500 | _ | _ | |a Waiting for fulltext |
| 520 | _ | _ | |a Eukaryotic tRNA guanine transglycosylase (TGT) is an RNA-modifying enzyme which catalyzes the base exchange of the genetically encoded guanine 34 of tRNAsAsp,Asn,His,Tyr for queuine, a hypermodified 7-deazaguanine derivative. Eukaryotic TGT is a heterodimer comprised of a catalytic and a non-catalytic subunit. While binding of the tRNA anticodon loop to the active site is structurally well understood, the contribution of the non-catalytic subunit to tRNA binding remained enigmatic, as no complex structure with a complete tRNA was available. Here, we report a cryo-EM structure of eukaryotic TGT in complex with a complete tRNA, revealing the crucial role of the non-catalytic subunit in tRNA binding. We decipher the functional significance of these additional tRNA-binding sites, analyze solution state conformation, flexibility, and disorder of apo TGT, and examine conformational transitions upon tRNA binding. |
| 536 | _ | _ | |a 6G3 - PETRA III (DESY) (POF4-6G3) |0 G:(DE-HGF)POF4-6G3 |c POF4-6G3 |f POF IV |x 0 |
| 536 | _ | _ | |a DFG project G:(GEPRIS)390729940 - EXC 2067: Multiscale Bioimaging: Von molekularen Maschinen zu Netzwerken erregbarer Zellen (390729940) |0 G:(GEPRIS)390729940 |c 390729940 |x 1 |
| 536 | _ | _ | |a EditMHC - How MHC-I editing complexes shape the hierarchical immune response (789121) |0 G:(EU-Grant)789121 |c 789121 |f ERC-2017-ADG |x 2 |
| 536 | _ | _ | |a SFB 860 Z01 - Zentrale Einrichtung für Kristallisationsexperimente (Z01) (174684579) |0 G:(GEPRIS)174684579 |c 174684579 |x 3 |
| 536 | _ | _ | |a SFB 860 A02 - Kristallographische Studien an molekularen Motoren des Spleißosoms (A02) (174570643) |0 G:(GEPRIS)174570643 |c 174570643 |x 4 |
| 536 | _ | _ | |a SFB 1565 P09 - Strukturelle und mechanistische Analyse des nukleozytoplasmatischen Transports von RNAs (P09) (515953039) |0 G:(GEPRIS)515953039 |c 515953039 |x 5 |
| 542 | _ | _ | |i 2024-03-01 |2 Crossref |u https://www.elsevier.com/tdm/userlicense/1.0/ |
| 542 | _ | _ | |i 2024-03-01 |2 Crossref |u https://doi.org/10.15223/policy-017 |
| 542 | _ | _ | |i 2024-03-01 |2 Crossref |u https://doi.org/10.15223/policy-037 |
| 542 | _ | _ | |i 2024-03-01 |2 Crossref |u https://doi.org/10.15223/policy-012 |
| 542 | _ | _ | |i 2024-03-01 |2 Crossref |u https://doi.org/10.15223/policy-029 |
| 542 | _ | _ | |i 2024-03-01 |2 Crossref |u https://doi.org/10.15223/policy-004 |
| 588 | _ | _ | |a Dataset connected to CrossRef, Journals: bib-pubdb1.desy.de |
| 693 | _ | _ | |a PETRA III |f PETRA Beamline P12 |1 EXP:(DE-H253)PETRAIII-20150101 |0 EXP:(DE-H253)P-P12-20150101 |6 EXP:(DE-H253)P-P12-20150101 |x 0 |
| 700 | 1 | _ | |a Neumann, Piotr |0 P:(DE-H253)PIP1018208 |b 1 |
| 700 | 1 | _ | |a Sušac, Lukas |b 2 |
| 700 | 1 | _ | |a Da Vela, Stefano |0 P:(DE-H253)PIP1107235 |b 3 |
| 700 | 1 | _ | |a Graewert, Melissa |0 P:(DE-H253)PIP1016308 |b 4 |
| 700 | 1 | _ | |a Trowitzsch, Simon |0 P:(DE-H253)PIP1030002 |b 5 |
| 700 | 1 | _ | |a Svergun, Dmitri |0 P:(DE-H253)PIP1001422 |b 6 |
| 700 | 1 | _ | |a Tampé, Robert |b 7 |
| 700 | 1 | _ | |a Ficner, Ralf |0 P:(DE-H253)PIP1018222 |b 8 |e Corresponding author |
| 773 | 1 | 8 | |a 10.1016/j.str.2023.12.006 |b Elsevier BV |d 2024-03-01 |n 3 |p 316-327.e5 |3 journal-article |2 Crossref |t Structure |v 32 |y 2024 |x 0969-2126 |
| 773 | _ | _ | |a 10.1016/j.str.2023.12.006 |g Vol. 32, no. 3, p. 316 - 327.e5 |0 PERI:(DE-600)2031189-8 |n 3 |p 316-327.e5 |t Structure |v 32 |y 2024 |x 0969-2126 |
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| 913 | 1 | _ | |a DE-HGF |b Forschungsbereich Materie |l Großgeräte: Materie |1 G:(DE-HGF)POF4-6G0 |0 G:(DE-HGF)POF4-6G3 |3 G:(DE-HGF)POF4 |2 G:(DE-HGF)POF4-600 |4 G:(DE-HGF)POF |v PETRA III (DESY) |x 0 |
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