TY  - EJOUR
AU  - Mesen-Ramirez, Paolo
AU  - Fuchs, Gwendolin
AU  - Burmester, Jonas
AU  - Farias, Guilherme
AU  - Alape-Flores, Ana-María
AU  - Singla, Shamit
AU  - Alder, Arne
AU  - Cubillán-Marín, José
AU  - Castro-Peña, Carolina
AU  - Lemcke, Sarah
AU  - Sondermann, Holger
AU  - Prado, Mónica
AU  - Spielmann, Tobias
AU  - Wilson, Danny William
AU  - Gilberger, Tim
TI  - Malaria parasite HOPS/CORVET complexes are critical for endocytosis and invasion organelles function
M1  - PUBDB-2024-08046
PY  - 2024
AB  - The tethering complexes HOPS/CORVET are central for vesicular fusion through the eukaryotic endolysosomal system, but the functions of these complexes in the intracellular development of malaria parasites are unknown. Here we show that early inactivation of core HOPS/CORVET complex subunits in Plasmodium falciparum leads to developmental arrest and accumulation of cytosolic vesicles, indicating a role of HOPS/CORVET in parasite endocytosis and fusion of endosomes to the digestive vacuole membrane. Late inactivation of the core HOPS/CORVET subunits led to the mislocalization of luminal rhoptry and microneme proteins, and to a severe defect in merozoite invasion. Ultra-expansion microscopy revealed a reduced rhoptry volume and the accumulation of numerous vesicles, further supporting a role of HOPS/CORVET in protein trafficking to the apical organelles. Malaria parasites have therefore repurposed HOPS/CORVET to perform dual functions consistent with a canonical endocytosis pathway for delivery of host cell material to the digestive vacuole in trophozoite stages and a parasite specific role in trafficking of protein cargo to the apical organelles required for invasion in schizont stages. 
LB  - PUB:(DE-HGF)25
DO  - DOI:10.1101/2024.10.04.616647
UR  - https://bib-pubdb1.desy.de/record/619955
ER  -