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000619955 1001_ $$0P:(DE-H253)PIP1100295$$aMesen-Ramirez, Paolo$$b0
000619955 245__ $$aMalaria parasite HOPS/CORVET complexes are critical for endocytosis and invasion organelles function
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000619955 520__ $$aThe tethering complexes HOPS/CORVET are central for vesicular fusion through the eukaryotic endolysosomal system, but the functions of these complexes in the intracellular development of malaria parasites are unknown. Here we show that early inactivation of core HOPS/CORVET complex subunits in Plasmodium falciparum leads to developmental arrest and accumulation of cytosolic vesicles, indicating a role of HOPS/CORVET in parasite endocytosis and fusion of endosomes to the digestive vacuole membrane. Late inactivation of the core HOPS/CORVET subunits led to the mislocalization of luminal rhoptry and microneme proteins, and to a severe defect in merozoite invasion. Ultra-expansion microscopy revealed a reduced rhoptry volume and the accumulation of numerous vesicles, further supporting a role of HOPS/CORVET in protein trafficking to the apical organelles. Malaria parasites have therefore repurposed HOPS/CORVET to perform dual functions consistent with a canonical endocytosis pathway for delivery of host cell material to the digestive vacuole in trophozoite stages and a parasite specific role in trafficking of protein cargo to the apical organelles required for invasion in schizont stages. 
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000619955 7001_ $$00000-0001-9294-6984$$aFuchs, Gwendolin$$b1
000619955 7001_ $$aBurmester, Jonas$$b2
000619955 7001_ $$0P:(DE-H253)PIP1104460$$aFarias, Guilherme$$b3
000619955 7001_ $$aAlape-Flores, Ana-María$$b4
000619955 7001_ $$aSingla, Shamit$$b5
000619955 7001_ $$0P:(DE-H253)PIP1084746$$aAlder, Arne$$b6
000619955 7001_ $$00009-0000-3171-7774$$aCubillán-Marín, José$$b7
000619955 7001_ $$00000-0002-6878-4951$$aCastro-Peña, Carolina$$b8
000619955 7001_ $$0P:(DE-H253)PIP1084745$$aLemcke, Sarah$$b9
000619955 7001_ $$0P:(DE-H253)PIP1093629$$aSondermann, Holger$$b10
000619955 7001_ $$aPrado, Mónica$$b11
000619955 7001_ $$00000-0002-3968-4601$$aSpielmann, Tobias$$b12
000619955 7001_ $$aWilson, Danny William$$b13
000619955 7001_ $$0P:(DE-H253)PIP1023784$$aGilberger, Tim$$b14$$eCorresponding author
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